Glypican-3 抗体 (YA5609)

Glypican-3 Antibody (YA5609) is a mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Glypican-3. It can be applicated for IHC-P, WB, ICC/IF, ELISA assays, in the background of human.

Glypican-3 是一种细胞表面蛋白聚糖 (PubMed:14610063)。当通过 GPI 锚定在细胞表面时，它通过竞争 hedgehog 受体 PTC1 与 hedgehog 蛋白的结合来负调控 hedgehog 信号通路 (By similarity)。与 hedgehog 蛋白 SHH 结合后，会触发复合物的内吞作用及随后的溶酶体降解 (By similarity)。此外，Glypican-3 通过结合 Wnt 受体 Frizzled 并刺激其与 Wnt 配体的结合，正调控经典 Wnt 信号通路 (PubMed:16227623，PubMed:24496449)，同时也正调控非经典 Wnt 信号通路 (By similarity)。它还能与 CD81 结合，减少游离 CD81 与转录抑制因子 HHEX 的结合，导致 HHEX 核转位和转录抑制 (By similarity)。此外，Glypican-3 抑制 DPP4 的二肽基肽酶活性 (PubMed:17549790)，并在肢体模式形成和骨骼发育中通过调控细胞对 BMP4 的响应发挥作用 (By similarity)。它还调节生长因子 BMP2、BMP7 和 FGF7 对肾分支形态发生的影响 (By similarity)，是冠状动脉血管发育所必需的 (By similarity)，并在原肠胚形成过程中调控细胞运动 (By similarity)。

P51654

Predicted band size: 66 kDa; Observed band size: 58 kDa

Protein G

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

IHC: 1:200-1000; WB: 1:500-2000; ICC/IF: 1:100-500; ELISA: 1:1000-5000

IHC-P, WB, ICC/IF, ELISA

液体

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Synthesized peptide derived from human Glypican-3(GPC3) AA range: 400-500