2. 抗体
  3. 一抗
  4. 单克隆抗体 重组抗体 流式抗体
  5. Histone H2B 抗体 (YA378)

Histone H2B 抗体 (YA378)

目录号: HY-P80163
COA 抗体使用指南 技术支持

Histone H2B Antibody 是一个非偶联、分子量约 14 kDa、兔源、抗 Histone H2B 单克隆抗体。它可用于人、小鼠、大鼠背景下 WB、ICC/IF、IHC-P、IP、FC 实验，且不带标记。

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规格 价格 是否有货 数量
10 μL ￥470 In-stock
50 μL ￥1220 In-stock
100 μL ￥2000 In-stock
250 μL   询价  

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Top Publications Citing Use of Products

【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

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描述

Histone H2B Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 14 kDa, targeting to Histone H2B. It can be used for WB,ICC/IF,IHC-P,IP,FC assays with tag free, in the background of Human, Mouse, Rat.
标签

Free
基因 ID
蛋白数据库
中文名
Histone H2B 抗体
同用名
H2B GL105 antibody; H2B histone family member O antibody; H2B histone family member S antibody; H2B.1 antibody; H2B.1 B antibody; H2B.b antibody; H2B.c antibody; H2B.d antibody; H2B.e antibody; H2B.f antibody; H2B.j antibody; H2B.q antibody; H2B/b antibody; H2B/c antibody; H2B/d antibody; H2B/e antibody; H2B/f antibody; H2B/j antibody; H2B/o antibody; H2B/q antibody; H2BFB antibody; H2BFC antibody; H2BFD antibody; H2BFE antibody; H2BFF antibody; H2BFJ antibody; H2BFO antibody; H2BFQ antibody; H2BFS antibody; HIRIP2 antibody; HIST1H2BB antibody; HIST1H2BD antibody; HIST1H2BH antibody; HIST1H2BL antibody; HIST1H2BM antibody; HIST1H2BN antibody; HIST2H2BE antibody; Histone H2B antibody; Histone H2B type 1 B antibody; Histone H2B type 1 D antibody; Histone H2B type 1 H antibody; Histone H2B type 1 L antibody; Histone H2B type 1 M antibody; Histone H2B type 1 N antibody; Histone H2B type 2 E antibody; histone protein antibody
分子量

Predicted band size: 14 kDa
纯度

Protein A affinity purified.
亚细胞定位

Nucleus, Chromosome.
偶联

Non-conjugated
修饰

Unmodified
RRID
研究领域

Epigenetics and Nuclear Signaling
产品类别

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
克隆性

Recombinant, Monoclonal
宿主

Rabbit
反应物种

Human, Mouse, Rat
推荐稀释比例

WB: 1:500-1:2,000 ;ICC/IF: 1:100-1:500 ;IHC-P: 1:50-1:5,000 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

  • Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Histone H2B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80163, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg)using Histone H2B (HY-P80163) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80163, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Histone H2B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80163, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunocytochemistry analysis of A431 cells labeling Histone H2B with Histone H2B antibody (HY-P80163) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H2B antibody (HY-P80163) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Western blot analysis of extracts from Hela (lane 2(20μg) , Hela (lane 3(20μg),using Histone H2B Antibody (HY-P80163). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
应用

WB, ICC/IF, IHC-P, IP, FC
性状

液体
组分

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
同型

IgG
敏感性

Endogenous
免疫原

Synthetic peptide corresponding to Human Histone H2B.AA range:110 to the C-terminus .
数据库

Entrez Gene: 337874 Human ; 54145 Human ; 8349 Human ; 104021 Mouse ; 64647 Rat

SwissProt: O60814 Human ; P06899 Human ; P23527 Human ; P33778 Human ; P57053 Human ; P58876 Human ; P62807 Human ; Q16778 Human ; Q5QNW6 Human ; Q8N257 Human ; Q93079 Human ; Q99877 Human ; Q99879 Human ; Q99880 Human ; P10854 Mouse ; Q64475 Mouse ; Q00729 Rat

OMIM: 615045 Human

文件资料

COA (193 KB)

抗体使用指南 (1083 KB)

Histone H2B Antibody (YA378) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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