Histone H3 (acetyl K56) 抗体 (YA374)

Histone H3 (acetyl K56) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 15 kDa, targeting to Histone H3 (acetyl K56). It can be used for WB,ICC/IF,IHC-P,ChIP,CUT&Tag-seq assays with tag free, in the background of Human, Mouse.

组蛋白 H3 是核小体的基本组成部分，在将 DNA 包裹和压缩到染色质的复杂过程中发挥着关键作用，这反过来又限制了 DNA 进入需要 DNA 作为模板的细胞机器。这种组蛋白及其对应物在关键细胞功能中发挥着关键作用，包括转录调节、DNA 修复、DNA 复制和染色体稳定性的维持。DNA 可及性的调节涉及翻译后修饰（统称为组蛋白密码）的复杂相互作用，以及核小体的动态重塑。核小体本身包含组蛋白八聚体，由 H2A、H2B、H3 和 H4 各两个分子组成，组装成一个 H3-H4 异四聚体和两个 H2A-H2B 异二聚体。这种八聚体有效地包裹了大约 147 个碱基对的 DNA，证明了它在组织染色质结构和促进关键基因组过程中不可或缺的作用。此外，组蛋白 H3 与各种细胞成分（例如 TONSL、CHAF1A、CHAF1B、MCM2 和 DNAJC9）相互作用，进一步促进其多方面功能。

Free

P68431

Predicted band size: 15 kDa; Observed band size: 15 kDa

Protein A affinity purified.

Nucleus, Chromosome, Nucleosome core.

Non-conjugated

Acetylated

AB_3102328

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:500-1:2,000 ;ICC/IF: 1:50-1:200 ;IHC-P: 1:50-1:800

• 
  Western blot analysis of extracts from Hela(lane 2(20μg),C6(lane 3(20μg) and NIH/3T3(lane 4(20ug) using Histone H3(HY-P80170 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling Histone H3 (acetyl K56) with Histone H3 (acetyl K56) Antibody (HY-P80170) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H3 (acetyl K56) Antibody (HY-P80170) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling Histone H3 (acetyl K56) with Histone H3 (acetyl K56) Antibody (HY-P80170) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H3 (acetyl K56) Antibody (HY-P80170)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded rat kidney tissue using Histone H3 (acetyl K56) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded rat kidney tissue using Histone H3 (acetyl K56) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, ChIP

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Histone H3.AA range:31-80.