Human IgM 抗体 (YA1062)

Human IgM Antibody (YA1062) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Human IgM.

Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region related gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of related genes: V related genes (see MIM 147070), joining (J) related genes (see MIM 147010), and diversity (D) related genes (see MIM 146910). The C region related genes are clustered downstream of the V region related genes within the heavy chain locus on chromosome 14. The IGHM related gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region related genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).

P01871

Predicted band size: 49 kDa; Observed band size: 75 kDa

Affinity Purified

Non-conjugated

Unmodified

Immunology

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

IHC: 1:50-1:100; ELISA: 1:10000

ELISA, IHC-P

液体

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2a

Endogenous

Purified recombinant full length of human IgM heavy chain protein expressed in E.coli.