Integrin alpha 5 抗体 (YA339)

Integrin alpha 5 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 115 kDa, targeting to Integrin alpha 5. It can be used for WB,ICC/IF,IHC-P,IP,FC assays with tag free, in the background of Human, Mouse.

Integrin alpha 5/beta 1 (ITGA5:ITGB1) 是一种受体，主要识别配体中的 R-G-D 序列，并与 fibronectin 和 fibrinogen 结合。它通过不同于经典配体结合位点（site 1）的位点（site 2）与 PLA2G2A 结合，从而诱导 integrin 构象变化并增强配体与 site 1 的结合。此外，ITGA5:ITGB1 作为 fibrillin-1 (FBN1) 和 fibronectin (FN1) 的受体，介导 R-G-D 依赖的细胞粘附。它还作为 IL1B 的受体，其结合对 IL1B 信号传导至关重要。by similar：ITGA5:ITGB3 是 soluble CD40LG 的受体，为 CD40/CD40LG 信号传导所需。微生物感染方面，ITGA5:ITGB1 可作为 Human metapneumovirus 的受体；ITGA2:ITGB1 则作为 Human parvovirus B19 的受体；在 HIV-1 感染中，与病毒 Tat 蛋白的相互作用可能促进 Kaposi's sarcoma 病变中的血管生成。

Free

P08648

Predicted band size: 115 kDa;Observed band size: 140/19 kDa

Protein A affinity purified.

Membrane, Cell junction, Cell surface.

Non-conjugated

Unmodified

AB_3102500

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1,000-1:2,000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

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  Western blot analysis of extracts from Hela (lane 2(20μg), NIH/3T3 (lane 3(20μg), A549 (lane 4(20μg) using Integrin alpha 5 Antibody (HY-P80416) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80416, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
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  Immunocytochemistry analysis of NIH3T3 cells labeling Integrin alpha 5 with Integrin alpha 5 Antibody (HY-P80416) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin alpha 5 Antibody (HY-P80416)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling Integrin alpha 5 with Integrin alpha 5 Antibody (HY-P80416)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin alpha 5 Antibody (HY-P80416) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue using Integrin alpha 5 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80416, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue using Integrin alpha 5 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80416, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Flow cytometric analysis of 1X10^6 Jurkat cells labeling Integrin alpha 5 Antibody (HY-P80416, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

WB, ICC/IF, IHC-P, IP, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Integrin alpha 5.The exact sequence is proprietary to MCE.