MC1R 抗体 (YA2019)

MC1R Antibody (YA2019) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MC1R.

MC1R-VLP 作为 MSH（α、β 和 γ）和 ACTH 的受体，通过腺苷酸的激活调节真黑素（黑色/棕色）和褐黑素（红色/黄色）的产生，在黑色素生成中发挥至关重要的作用通过G蛋白环化酶。该受体与 MGRN1 相互作用，通过与 GNAS 结合竞争来抑制激动剂诱导的 cAMP 产生，尽管它不经历 MGRN1 介导的泛素化。此外，MC1R-VLP 与 OPN3 相互作用，导致 MC1R 介导的 cAMP 信号传导减少，从而减少黑素细胞中黑色素的产生。这些相互作用凸显了 MC1R-VLP 介导的黑素生成的复杂调节机制。

Q01726

Predicted band size: 35 kDa; Observed band size: 35 kDa

Affinity Chromatography

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IHC: 1/50-1/100

• 
  Western blot analysis was performed on extracts from A375 (lane 1, 15 μg), MCF-7 (lane 2, 15 μg), HepG2 (lane 3, 15 μg), and U2OS (lane 4, 15 μg) using MC1R Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human MC1 Receptor