MRP1 抗体 (YA1583)

MRP1 Antibody (YA1583) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MRP1.

MRP1 介导有机阴离子和药物从细胞质中的输出。它通过 ATP 依赖性转运谷胱甘肽 (glutathione) 及其结合物、白三烯 C4 (leukotriene C4)、雌二醇-17-β-葡萄糖醛酸苷 (estradiol-17-beta-o-glucuronide)、甲氨蝶呤 (methotrexate)、抗病毒药物和其他外源性物质发挥作用。此外，MRP1 通过减少药物在细胞内的积累以及介导 ATP 和 GSH 依赖性药物外排来赋予抗癌药物耐药性。它还能以低效率水解 ATP，并独立于肥大细胞脱颗粒催化鞘氨醇 1-磷酸 (sphingosine 1-phosphate) 的分泌。通过允许白三烯 C4 从合成细胞中输出，MRP1 参与炎症反应 (by similarity)。此外，它介导 ATP 依赖性、GSH 非依赖性的环状 GMP-AMP (cGAMP) 输出，从而通过限制细胞内 cGAMP 浓度负调控 cGAS-STING 通路。在淋巴组织和基质区室中，MRP1 输出 S-香叶基香叶基谷胱甘肽 (GGG)，与 GGT5 共同在淋巴组织中建立 GGG 梯度，以将 P2RY8 阳性淋巴细胞定位在淋巴滤泡的生发中心，并限制其从血管向骨髓实质的趋化迁移 (by similarity)。在极化上皮细胞中，MRP1 介导 GSH 结合的 R-和 S-前列腺素 A2 非对映异构体的基底外侧输出。

P33527

Predicted band size: 172 kDa;Observed band size: 250 kDa

Affinity Chromatography

Non-conjugated

Unmodified

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; FC: 1:50-1:100

• 
  Western blot analysis was performed on extracts from A549 (lane 1, 20 μg), K562 (lane 2, 20 μg), HepG2 (lane 3, 20 μg), A431 (lane 4, 20 μg), 3T3 (lane 5, 20 μg), and Hela (lane 6, 20 μg) using MRP1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, FC

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human MRP1