NUMA1 抗体 (YA6350)

NUMA1 Antibody (YA6350) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NUMA1. It can be applicated for WB, IHC-P, IHC-F, ICC/IF, IF-Tissue, ELISA, FC assays, in the background of human, mouse, rat.

NUMA1 是一种微管 (MT) 结合蛋白，在有丝分裂细胞分裂过程中参与纺锤体极的形成与维持，以及染色体的排列和分离。其主要功能是将微管的负端锚定在纺锤体极，这对纺锤体极的建立和维持至关重要。NUMA1 还通过依赖 dynein-dynactin 复合物的方式，在中期和有丝分裂后期调控纺锤体的定向和伸长。在中期，NUMA1 与 GPSM2 和 G(i) alpha 蛋白形成三元复合物，调控 dynein-dynactin 复合物在纺锤体极上方皮层区域的招募和锚定，从而确保纺锤体的正确方向。在有丝分裂后期，NUMA1 通过直接结合磷脂酰肌醇 4,5-二磷酸 (PI(4,5)P2)，促进 dynein-dynactin 复合物在极皮层区域的积累，参与纺锤体伸长和染色体分离的调控。此外，NUMA1 还能与其他聚阴离子磷脂酰肌醇（如 PIP、LPA 和 PIP3）结合。它还在不对称细胞分裂中调控纺锤体的定向，参与无星纺锤体的组装，并正调控 TNKS 蛋白在纺锤体极的定位。NUMA1 是核基质的主要成分之一，可能在非有丝分裂过程中发挥结构作用。此外，NUMA1 对表皮分化和毛囊形态发生也是必需的 (by similar)。

Q14980

Predicted band size: 238 kDa; Observed band size: 238 kDa

affinity purified.

Nucleus matrix. Chromosome. Cytoplasm, cytoskeleton, spindle pole. Note=Resides in the nuclear matrix during interphases. Dissociates from condensing chromosomes during early prophase, and relocates to the spindle poles via dynein/dynamin association, it remain there until the anaphase onset. Before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. Isoform Numa-m: Cytoplasm. Note=Mainly clustered at the centrosomal region. Isoform Numa-s: Cytoplasm. Note=Mainly clustered at the centrosomal region.

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-2000; IHC-P: 1:100-500; IHC-F: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500; ELISA: 1:5000-20000; Flow-Cyt: 1μg/Test

WB, IHC-P, IHC-F, ICC/IF, IF-Tissue, ELISA, FC

液体

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

KLH conjugated synthetic peptide derived from human NUMA1