PARP2 抗体

PARP2 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 66 kDa, targeting to PARP2. It can be used for WB,FC assays with tag free, in the background of Human.

Free

Predicted band size: 66 kDa

Protein A affinity purified.

Chromosome, Nucleus.

Non-conjugated

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Rabbit

Human

SwissProt: Q9UGN5 Human;SwissProt: O88554 Mouse;SwissProt: G3V749 Rat

WB: 1:500-1:1,000 ;FC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela(lane 2(20μg) or lane 3(40μg)) and Raji(lane 4(20μg) or lane 5(40μg)), using PARP2 (HY-P80265) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80265, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Synthetic peptide corresponding to Human PARP2.AA range:484-583.