PCBP2 抗体 (YA1543)

PCBP2 Antibody (YA1543) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PCBP2.

The protein encoded by The related gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, The protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. The multiexon structural mRNA is thought to be retrotransposed to related generate PCBP-1, an intronless related gene with functions similar to that of PCBP2. The related gene and PCBP-1 have paralogous related genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire related genes. The related gene also has two processed pseudorelated genes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for The related gene.

Q15366

Predicted band size: 39 kDa; Observed band size: 35-45 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3103899

Microbiology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Mouse, Rat

WB: 1:500-1:1000; IP: 1:20

• 
  Western blot analysis of extracts from C6 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Hela (lane 4(20μg) using PCBP2 (HY-P81798) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IP

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human PCBP2