Phospho-AKT (Thr308) 抗体

Phospho-AKT (Thr308) Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 56 kDa, targeting to Phospho-AKT (Thr308). It can be used for ICC/IF,WB,IHC-F,IHC-P,ELISA assays with tag free, in the background of Human, Mouse, Rat.

AKT：该基因编码人类 AKT 丝氨酸-苏氨酸蛋白激酶家族的三个成员之一，通常称为蛋白激酶 B α、β 和γ。这些高度相似的 AKT 蛋白都有一个 N 端 pleckstrin 同源结构域、一个丝氨酸/苏氨酸特异性激酶结构域和一个 C 端调节结构域。这些蛋白质被磷酸肌醇 3-激酶 (PI3K) 磷酸化。AKT/PI3K 是许多信号通路的关键组成部分，这些信号通路涉及膜结合配体的结合，例如受体酪氨酸激酶、G 蛋白偶联受体和整合素连接激酶。因此，这些 AKT 蛋白调节多种细胞功能，包括正常细胞和恶性细胞中的细胞增殖、存活、代谢和血管生成。磷脂酰肌醇 4,5-二磷酸 (PIP2) 被 PI3K 磷酸化后，AKT 蛋白被磷脂酰肌醇 3,4,5-三磷酸 (PIP3) 募集到细胞膜上。苏氨酸残基 308 和丝氨酸残基 473 的后续磷酸化是完全激活该基因编码的 AKT1 蛋白所必需的。其他残基的磷酸化也会发生，例如，响应胰岛素生长因子-1 和表皮生长因子。蛋白磷酸酶通过使 AKT 或 PIP3 去磷酸化而充当 AKT 蛋白的负调节剂。PI3K/AKT 信号通路对肿瘤细胞存活至关重要。存活因子可以通过激活 AKT1 以转录非依赖性方式抑制细胞凋亡，然后磷酸化和灭活细胞凋亡机制的组分。AKT 蛋白还参与哺乳动物雷帕霉素靶标 (mTOR) 信号通路，该通路控制真核翻译起始因子 4F (eIF4E) 复合物的组装，并且除了响应来自生长因子和细胞因子的细胞外信号外，该通路在许多癌症。该基因的突变与多种类型的癌症和组织过度生长有关，包括变形杆菌综合征和考登综合征 6，以及乳腺癌、结直肠癌和卵巢癌。已发现该基因的多个选择性剪接转录物变体。[由 RefSeq 提供，2020 年 7 月]

Free

P31749

Predicted band size: 56 kDa; Observed band size: 56 kDa

affinity purified

Non-conjugated

Phosphorylated

AB_3662913

Signal Transduction

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000 ; IHC: 1:50-1:100 ; IF: 1:50-1:200 ; ELISA: 1:10000

• 
  Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Phospho-AKT (Thr308) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Phospho-AKT (Thr308) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ICC/IF, WB, IHC-F, IHC-P, ELISA

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Thr308 of Human Akt.The exact sequence is proprietary to MCE.