Phospho-CDC37 (Ser13) 抗体 (YA219)

Phospho-CDC37 (Ser13) Antibody (YA219) is a non-conjugated and Rabbit origined monoclonal antibody about 44 kDa, targeting to Hsp90 co-chaperone Cdc37 (CDC37). It can be used for WB,IP assays in the background of Human, Mouse, Rat.

Hsp90 co-chaperone Cdc37 (CDC37)：该基因编码的蛋白质与酿酒酵母细胞分裂周期控制蛋白 Cdc 37 高度相似。该蛋白是一种分子伴侣，在细胞信号转导中具有特定功能。它已被证明与 Hsp90 和多种蛋白激酶（包括 CDK4、CDK6、SRC、RAF-1、MOK 以及 eIF2 α 激酶）形成复合物。它被认为在将 Hsp90 导向其目标激酶方面起着关键作用。[由 RefSeq 提供，2008 年 7 月]

11140

Predicted band size: 44 kDa

Non-conjugated

Phosphorylated

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

Q16543

WB: 1:500-1:1000 ;IP: 1:20

• 
  Western blot analysis of extracts from Hela(lane 2(20μg) ) and Hela(lane 3(20μg), Calyculin A treated (HY-18983, 50nm, 3hrs) as indicated, using Phospho-CDC37 (Ser13) (HY-P80962) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80962, 1/1000) and Loading control antibody (CDC37, HY-P80960, 1/1000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB, IP

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser13 of Human Cdc37.The exact sequence is proprietary to MCE.