Phospho-Histone H2A.X (Ser139) 抗体 (YA190)

Phospho-Histone H2A.X (Ser139) Antibody (YA190) is a non-conjugated and Rabbit origined monoclonal antibody about 15 kDa, targeting to Phospho-Histone H2A.X(S139). It can be used for WB,IHC-P,ICC/IF, IF-Tissue assays with tag free, in the background of Human, Mouse, Rat.

Free

P16104

Predicted band size: 15 kDa

Protein A affinity purified.

Nucleus, Chromosome

Non-conjugated

Phosphorylated

AB_3102537

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat, Monkey

WB: 1:500 ;IHC-P: 1:50-1:500;ICC/IF:1:2,000;IF-Tissur: 1:500

• 
  Western blot analysis of extracts from Hela(lane 2(20μg) , HEK293(lane 3(20μg) and C6(lane 4(20ug) using Phospho-Histone H2A.X(HY-P80463 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Phospho-Histone H2A.X Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Phospho-Histone H2A.X Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, IF-Tissue

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser139 of Human Histone H2AX.The exact sequence is proprietary to MCE.