Phospho-JNK1/2/3 (Thr183) 抗体

Phospho-JNK1/2/3 (Thr183) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-JNK1/2/3 (Thr183).

JNK1 是一种丝氨酸/苏氨酸蛋白激酶，复杂地调节多种细胞过程，包括细胞增殖、分化、迁移、转化和程序性细胞死亡。应激激活蛋白激酶/c-Jun N 末端激酶 (SAP/JNK) 信号通路在促炎细胞因子或身体应激等细胞外因素的刺激下被激活，涉及双特异性激酶对 JNK1 的磷酸化和激活MAP2K4/MKK4 和 MAP2K7/MKK7。在随后的级联反应中，JNK1 磷酸化关键转录因子，特别是 AP-1 的成分，例如 JUN、JDP2 和 ATF2，从而调节 AP-1 转录活性。JNK1 还通过磷酸化复制许可因子 CDT1，破坏其与组蛋白 H4 乙酰化酶 HBO1 的相互作用，对复制起始进行调节控制。该事件阻碍了复制起点所需的乙酰化。此外，JNK1 通过磷酸化关键调节因子（包括 p53/TP53 和 Yes 相关蛋白 YAP1），在细胞凋亡诱导中发挥关键作用。它参与多种细胞途径，包括对 T 细胞分化、红系细胞存活、自噬激活和神经元微管动力学调节的贡献。JNK1 广泛的底物库包括热休克因子蛋白 4 (HSF4)、脱乙酰酶 SIRT1、ELK1、E3 连接酶 ITCH、生物钟调节器 CLOCK-BMAL1 和热休克转录因子 HSF1 等。值得注意的是，JNK1 同工型表现出不同的结合模式，β-1 优先与 c-Jun 结合，而 alpha-1、alpha-2 和 beta-2 显示出与 c-Jun 和 ATF2 相似的低水平结合，尽管磷酸化不同异构体的效率保持一致。

Free

P45983 / P45984 / P53779

Predicted band size: 48 kDa; Observed band size: 46/54 kDa

affinity purified

Non-conjugated

Phosphorylated

AB_3102216

Signal Transduction

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; IP: 1:20; FC: 1:50-1:100

• 
  Western blot analysis was performed on extracts from 293T+CA (lane 1, 20 μg), and 3T3+CA (lane 2, 20 μg) using Phospho-JNK (Thr183) Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P, ICC/IF, IP, FC

液体

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Thr183 of Human Phospho-JNK1/2/3.The exact sequence is proprietary to MCE.