Retinoic Acid Receptor alpha 抗体 (YA096)

Retinoic Acid Receptor alpha Antibody (YA096) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Retinoic Acid Receptor alpha.

Retinoic Acid Receptor alpha (RARα) 是一种视黄酸受体，可与全反式或 9-顺式视黄酸结合，通过异源二聚体形式（如与 RXR 形成 RXR/RAR 异源二聚体）结合靶标反应元件（如 DR1-DR5 类型的 RARE 序列 5'-AGGTCA-3'），调控多种生物学过程中的基因表达。无配体时，RXR-RAR 异源二聚体与包含转录辅阻遏物的多蛋白复合物结合，导致组蛋白去乙酰化、染色质浓缩和转录抑制；配体结合后，辅阻遏物解离并与辅激活物结合，启动转录激活。与组蛋白去乙酰化酶形成复合物可能抑制 RARE 结合并导致转录抑制，而转录因子 KLF2 可能支持 RARE 结合和转录激活。RARA 在视黄酸诱导的精子发生过程中生殖细胞发育调控中起关键作用（by similar），并支持早期精母细胞在减数分裂前期的存活（by similar）。与 RARG 协同调控骨骼生长、基质稳态和生长板功能（by similar）。与 RXRA 共同正调控 microRNA-10a 表达，抑制血管内皮细胞中 GATA6/VCAM1 信号对脉动剪切应力的响应；与 HDAC3/5/7 结合时抑制 microRNA-10a，促进炎症反应。

Free

P10276

Predicted band size: 51 kDa; Observed band size: 75 kDa

Protein A affinity purified.

Nucleus, Cytoplasm.

Non-conjugated

Unmodified

AB_3102104

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:50-1:2000; FC: 1:50-1:100

• 
  Western blot analysis of extracts from NIH/3T3(lane2(20μg) , HEK293(lane 3(20μg) and MCF-7(lane 4(20ug) using Retinoic Acid Receptor alpha Antibody (HY-P80308) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Retinoic Acid Receptor alpha.AA range:1-50.