SAM68 抗体 (YA2136)

SAM68 Antibody (YA2136) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SAM68.

SAM68 是一种 RNA 结合蛋白，参与多种受体系统（如 T 细胞、瘦素和胰岛素受体）的招募和酪氨酸磷酸化。磷酸化后，它通过结合含 SH2 和 SH3 结构域的蛋白质，在信号转导级联中充当适配蛋白。在细胞周期中，SAM68 参与 G2-M 期进程，并通过竞争性结合 CBP 抑制 CBP 依赖性转录激活。此外，它作为 mRNA 稳定性和/或翻译速率的潜在调节因子，介导 mRNA 核输出，并促进含组成型转运元件（CTE）的 mRNA 与大聚核糖体的结合及翻译起始。然而，有研究表明 SAM68 不参与未剪接 CTE 含 RNA 的核质输出。SAM68 还调控选择性剪接，影响 mRNA 剪接位点选择和外显子包含，特异性结合含 5'-[AU]UAA-3' 双部分基序（间隔超过 15 个核苷酸）的 RNA 及 poly(A)。在 Ras 通路依赖的方式下调控 CD44 选择性剪接，并与 HNRNPA1 协同调控 BCL2L1（促进 Bcl-X(S) 亚型生成）和 SMN1 的选择性剪接。在神经元活动依赖性方式下，与 KHDRBS2/SLIM-1 协同调控 NRXN1 和 NRXN3 在层粘连蛋白 G 样结构域 6 中的选择性剪接（含进化保守的 AS4 片段），但对 NRXN3 的调控表现为拮抗作用（By similarity）。亚型 3 仅在生长停滞细胞中表达，抑制 S 期进程。

Q07666

Predicted band size: 48 kDa; Observed band size: 68 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3104500

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200

• 
  Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using SAM68 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling SAM68 with SAM68 Antibody (HY-P82391) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with SAM68 Antibody (HY-P82391) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH3T3 cells labeling SAM68 with SAM68 Antibody (HY-P82391) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with SAM68 Antibody (HY-P82391)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using SAM68 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using SAM68 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human SAM68