STAT2 抗体 (YA057)

STAT2 Antibody (YA057) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STAT2.

STAT2 是一种信号转导和转录激活因子，介导 I 型干扰素（IFN-alpha 和 IFN-beta）的信号传导。当 I 型干扰素与细胞表面受体结合后，Jak 激酶（TYK2 和 JAK1）被激活，导致 STAT1 和 STAT2 的酪氨酸磷酸化。磷酸化的 STATs 二聚化并与 IRF9/ISGF3G 结合形成 ISGF3 转录因子复合物，进入细胞核。ISGF3 结合干扰素刺激反应元件（ISRE）激活干扰素刺激基因的转录，使细胞进入抗病毒状态。此外，STAT2 还通过招募 USP18 至 I 型干扰素受体亚基 IFNAR2 负反馈调节 I 型干扰素信号传导，从而减弱对 I 型干扰素的反应。STAT2 还通过调节 DNM1L 在 Ser-616 和 Ser-637 的磷酸化来调控线粒体分裂，分别激活和失活 DNM1L 的 GTPase 活性。

Free

P52630

Predicted band size: 98 kDa; Observed band size: 105 kDa

Protein A affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Unmodified

AB_3102442

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500; ICCUF: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:100

• 
  Western blot analysis of extracts from 293T(lane 1) and K562 (lane 2) and Ramos (lane 3) using STAT2 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, IHC-P, ICC/IF, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human STAT2.AA range:1-49.