1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. beta-1,4-Galactosyltransferase 1 (Y285L)

beta-1,4-Galactosyltransferase 1 (Y285L)  (Synonyms: B4GALT1 (Y285L))

目录号: HY-E70285
COA 产品使用指南 技术支持

beta-1,4-Galactosyltransferase 1 (Y285L) 可以酶促合成 LacdiNAc 基序。beta-1,4-Galactosyltransferase 1 (Y285L) 可以从 UDP-GalNAc 上转移 GalNAc。

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beta-1,4-Galactosyltransferase 1 (Y285L)

beta-1,4-Galactosyltransferase 1 (Y285L) Chemical Structure

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规格 价格 是否有货 数量
100 μg ¥4140
In-stock
1 mg 现货 询价
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Customer Review

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

beta-1,4-Galactosyltransferase 1 (Y285L) can enzymatic synthesis of the LacdiNAc motif. beta-1,4-Galactosyltransferase 1 (Y285L) can transfer of GalNAc from UDP-GalNAc[1].

体外研究
(In Vitro)

产品信息
本产品为重组 HEK293 cell 表达,C-terminal His Tagged,分子量 39.7 kDa,储存缓冲液组分为 20 mM Tris-HCl(PH7.4), 200 mM NaCl, 20% Glycerin,能有效转移 GalNAc 或者 GalNAz 等衍生物到末端 GlcNAc 残基,后者可以应用于抗体-药物偶联物 (ADC) 作为靶向癌症治疗等。

使用说明(可根据实验情况调整)
反应体系:
100 mM Tris-HCl (pH 7.5),6 mM MnCl2;
2、反应试剂:
1) UDP-GalNAz:抗体(当量比)= 300:1;
2) 酶添加量:酶/抗体=5%;
3) 按照上述条件配制反应液,37℃ 反应 48 小时,后置于 -15℃ 至 -25℃ 停止反应并进行样品检测。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

性状

液体

颜色

Colorless to light yellow

中文名称

β-1,4-半乳糖基转移酶 1 (Y285L)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料

参考文献

beta-1,4-Galactosyltransferase 1 (Y285L) 相关分类

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
beta-1,4-Galactosyltransferase 1 (Y285L)
目录号:
HY-E70285
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