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  3. BODIPY 505/515-8-C3-COOH

BODIPY 505/515-8-C3-COOH 是一种绿色荧光衍生物,可作为成像纳米绿藻脂滴的荧光染料。BODIPY 505/515-8-C3-COOH 可用于流式细胞术高通量筛选和细胞分选的研究。

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BODIPY 505/515-8-C3-COOH Chemical Structure

BODIPY 505/515-8-C3-COOH Chemical Structure

CAS No. : 878674-84-1

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Top Publications Citing Use of Products
  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

BODIPY 505/515-8-C3-COOH is a green fluorescing derivative, as a fluorescent dye for imaging lipid droplets in nannochloropsis. BODIPY 505/515-8-C3-COOH can be used for the research of flow cytometric high-throughput screening and cell sorting[1].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Cells:
1. Fresh N. oceanica cultures are diluted to ~4×106 cells/ml with ASW and kept at 22℃ prior to any treatment. Incubate the cells according to your normal protocol.
2. BODIPY 505/515 is dissolved in DMSO at 4 mg/ml and diluted with DMSO to different working stock concentrations.
3. Cell suspensions are supplemented with the appropriate BODIPY 505/515 working stock to a specific DMSO concentration between 2 and 10% (v/v) with final BODIPY concentrations between 0.8 and 4 μg/ml.
4. Pure DMSO was used for control treatments. 1 ml of fresh culture was diluted to ~4×106 cells/ml with ASW and stained with 6% DMSO and 1.2 μg/ml BODIPY for 15 min (non-stressed cultures) or with 10% DMSO and 1.6 μg/ml BODIPY for 36 min (stressed cultures).
5. Upon addition of the dye, samples were vortexed for 5 s and then incubated in the dark for 15 min before flow cytometric analysis, if not indicated otherwise.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

334.17

Formula

C17H21BF2N2O2

CAS 号
Emission (Em)

515

Excitation (Ex)

505

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献

BODIPY 505/515-8-C3-COOH 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
BODIPY 505/515-8-C3-COOH
目录号:
HY-D1581
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