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  3. Fluorescent Dye
  4. BODIPY 505/515-8-C3-COOH

BODIPY 505/515-8-C3-COOH 

目录号: HY-D1581 纯度: 99.92%
COA 产品使用指南 技术支持

BODIPY 505/515-8-C3-COOH 是一种绿色荧光衍生物，可作为成像纳米绿藻脂滴的荧光染料。BODIPY 505/515-8-C3-COOH 可用于流式细胞术高通量筛选和细胞分选的研究。

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BODIPY 505/515-8-C3-COOH Chemical Structure

BODIPY 505/515-8-C3-COOH Chemical Structure

CAS No. : 878674-84-1

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2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
5 mg ￥2200
In-stock
10 mg ￥3400
In-stock
25 mg 现货 询价
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

BODIPY 505/515-8-C3-COOH 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

BODIPY 505/515-8-C3-COOH is a green fluorescing derivative, as a fluorescent dye for imaging lipid droplets in nannochloropsis. BODIPY 505/515-8-C3-COOH can be used for the research of flow cytometric high-throughput screening and cell sorting[1].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Cells:
1. Fresh N. oceanica cultures are diluted to ~4×106 cells/ml with ASW and kept at 22℃ prior to any treatment. Incubate the cells according to your normal protocol.
2. BODIPY 505/515 is dissolved in DMSO at 4 mg/ml and diluted with DMSO to different working stock concentrations.
3. Cell suspensions are supplemented with the appropriate BODIPY 505/515 working stock to a specific DMSO concentration between 2 and 10% (v/v) with final BODIPY concentrations between 0.8 and 4 μg/ml.
4. Pure DMSO was used for control treatments. 1 ml of fresh culture was diluted to ~4×106 cells/ml with ASW and stained with 6% DMSO and 1.2 μg/ml BODIPY for 15 min (non-stressed cultures) or with 10% DMSO and 1.6 μg/ml BODIPY for 36 min (stressed cultures).
5. Upon addition of the dye, samples were vortexed for 5 s and then incubated in the dark for 15 min before flow cytometric analysis, if not indicated otherwise.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

BODIPY 505/515-8-C3-COOH 相关抗体:
分子量

334.17

Formula

C17H21BF2N2O2
CAS 号
性状

固体

颜色

Orange to red

Emission (Em)

515
Excitation (Ex)

505
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 100 mg/mL (299.25 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.9925 mL 14.9624 mL 29.9249 mL
5 mM 0.5985 mL 2.9925 mL 5.9850 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

BODIPY 505/515-8-C3-COOH 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9925 mL 14.9624 mL 29.9249 mL 74.8122 mL
5 mM 0.5985 mL 2.9925 mL 5.9850 mL 14.9624 mL
10 mM 0.2992 mL 1.4962 mL 2.9925 mL 7.4812 mL
15 mM 0.1995 mL 0.9975 mL 1.9950 mL 4.9875 mL
20 mM 0.1496 mL 0.7481 mL 1.4962 mL 3.7406 mL
25 mM 0.1197 mL 0.5985 mL 1.1970 mL 2.9925 mL
30 mM 0.0997 mL 0.4987 mL 0.9975 mL 2.4937 mL
40 mM 0.0748 mL 0.3741 mL 0.7481 mL 1.8703 mL
50 mM 0.0598 mL 0.2992 mL 0.5985 mL 1.4962 mL
60 mM 0.0499 mL 0.2494 mL 0.4987 mL 1.2469 mL
80 mM 0.0374 mL 0.1870 mL 0.3741 mL 0.9352 mL
100 mM 0.0299 mL 0.1496 mL 0.2992 mL 0.7481 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BODIPY 505/515-8-C3-COOH878674-84-1Fluorescent Dyegreen fluorescing derivativefluorescent dyenannochloropsis imagingflow cytometric high-throughput screeningcell sortingInhibitorinhibitorinhibit

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