BODIPY FL C12 

BODIPYFL C12 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the polar lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells^[1]. Maximum excitation/emission wavelength: 480/508 nm^[1].

使用方法
1. BODIPYFL C12 工作液的配制
1.1 制备储存液
用 382 μL 无水 DMSO 稀释 1mg BODIPYFL C12 去配制 10 mM 的储备液。
注：BODIPYFL C12 储存液建议分装后于 -20℃ 或 -80℃ 避光保存。
1.2 工作液的配制
用预热好的无血清细胞培养基或 PBS 稀释储存液，配制成 1-10 μM 的 BODIPYFL C12 工作液。
注：请根据实际情况调整 BODIPYFL C12 工作液浓度，且现用现配。
2. 细胞染色（悬浮细胞）
2.1 离心收集细胞，加入 PBS 洗涤两次，每次 5 min。细胞密度在 1×10⁶/mL
2.2 加入 1 mL BODIPYFL C12 工作液，室温孵育 5-30 min。
2.3 400 g，离心 3-4 min，弃去上清。
2.4 加入 PBS 洗涤细胞两次，每次 5 min。
2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，使用荧光显微镜或流式细胞仪进行观察。
3. 细胞染色（贴壁细胞）
3.1 将贴壁细胞培养于无菌盖玻片上。
3.2 从培养基中移出盖玻片，吸除多余培养基。
3.3 加入 100 μL 染料工作液，轻轻晃动使其完全覆盖细胞，孵育 5-30 min。
3.4 吸去染料工作液，用培养基洗 2-3 次，每次 5 min，使用荧光显微镜或流式细胞仪进行观察。

保存条件
-20℃ 避光保存一年。

注意事项
1. 请根据实际情况调整 BODIPYFL C12 工作液浓度与孵育时间。
2. 实验建议进行阳性对照，将对照组细胞与 30 μM 油酸孵育 8 h 后进行后续实验。
3. 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品。
4. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

418.33

C₂₃H₃₃BF₂N₂O₂

158757-79-0

516

495

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Kang ZB, et al. Fluopack screening platform for unbiased cellular phenotype profiling. Sci Rep. 2020 Feb 7;10(1):2097.  [Content Brief]

  [2]. Kamkaew A, et al. BODIPY dyes in photodynamic therapy. Chem Soc Rev. 2013 Jan 7;42(1):77-88.  [Content Brief]

  [3]. Bo Qiu, et al. BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Bio Protoc. 2016 Sep 5;6(17):e1912.  [Content Brief]

  [4]. Glende J, et al. Importance of cholesterol-rich membrane microdomains in the interaction of the S protein of SARS-coronavirus with the cellular receptor angiotensin-converting enzyme 2. Virology. 2008 Nov 25;381(2):215-21.  [Content Brief]