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  2. Fluorescent Dye
  3. BODIPY FL SSE

BODIPY FL SSE 是一种有效的荧光染料。BODIPY FL SSE 可用于标记蛋白质的伯胺 (R-NH2)、胺修饰的寡核苷酸以及其他含胺分子。BODIPY FL SSE 可以与生物分子上的伯胺反应,发出绿色荧光。(λex=502 nm,λem=511 nm)。

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BODIPY FL SSE Chemical Structure

BODIPY FL SSE Chemical Structure

CAS No. : 217190-17-5

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

BODIPY FL SSE is a potent fluorescent dye. BODIPY FL SSE is used to label the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. BODIPY FL SSE can reactive with primary amines on biomolecules to emit green fluorescence. (λex=502 nm, λem=511 nm)[1][2].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
BODIPY FL SSE assay (to quantify GAPDH)[1]:
1.Make a 2 mg/ mL stock of GAPDH (MW 37 kDa) in PBS (pH 7.4).
2.Add 54 nM of BODIPY-FL-SSE to 1 mL of the 2 mg/ mL (54 μM) GAPDH solution. Incubate the mixture for 4 h on ice.
3.Dialyze the reaction mixture against 500 ml of PBS, pH 7.4, in a 10,000 MW-cutoff dialysis cassette for 1 h at 4 °C. Change the buffer and dialyze again overnight. Change the dialysis buffer once more the following morning and dialyze further for 1 h.
4.Run the dialyzed sample through a Zeba desalting column at 1000 g for 2 min.
5.Analyze the BD-GAPDH spectrophotometrically in a quartz cuvette. Set the wavelength scan to 250-700 nm. Blank the instrument against PBS and add the BD-GAPDH to the cuvette.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

491.21

Formula

C18H17BF2N3NaO7S

CAS 号
Emission (Em)

512

Excitation (Ex)

501

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献

BODIPY FL SSE 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
BODIPY FL SSE
目录号:
HY-D1607
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