1. Cell Cycle/DNA Damage
  2. DNA Stain
  3. CellTracker Blue CMF2HC Dye

CellTracker Blue CMF2HC Dye 

目录号: HY-D1571 纯度: 99.78%
COA 产品使用指南

CellTracker Blue CMF2HC Dye 是一种蓝色荧光染料,可应用于双通道核酸测序,使用蓝色和紫色激发光 (450-460 nm/400-405nm 或 415-450 nm/480-525nm)。CellTracker Blue CMF2HC Dye 也可用于微生物抗生素敏感性的快速测定。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

CellTracker Blue CMF2HC Dye Chemical Structure

CellTracker Blue CMF2HC Dye Chemical Structure

CAS No. : 215868-45-4

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
1 mg ¥1140
In-stock
5 mg ¥3300
In-stock
10 mg ¥5000
In-stock
25 mg 现货 询价
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

CellTracker Blue CMF2HC Dye is a blue dye, can be used in two-channel nuclei acid sequencing, with blue and purple excitation light (450-460 nm/400-405nm or 415-450 nm/480-525nm). CellTracker Blue CMF2HC Dye can be used to rapid determination of antibiotic sensitivity of microorganisms[1][2].

体外研究
(In Vitro)

CellTracker Blue CMF2HC Dye is a blue dye that can be excitable by a blue light source having a wavelength of about 450-460 nm, is used as the first or the second detectable label described herein[1].
Blue/Violet Two-Channel Sequencing Methods[1]:
1.Contacting a primer polynucleotide/taiget polynucleotide complex with a mixture comprising one or more of a first type of nucleotide, a second type of nucleotide, a third type of nucleotide, and a fourth type of nucleotide, wherein the primer polynucleotide is complementary to at least a portion ofthe single stranded target polynucleotide;
2.Incorporating one type of nucleotide from the mixture to the primer polynucleotide to produce an extended primer polynucleotide (i.e., an extended primer polynucleotide/target polynucleotide complex);
3.Performing a first imaging event using a first excitation light source and collecting a first emission signal from the extended primer polynucleotide/target polynucleotide complex with a first emission filer;
4.Performing a second imaging event using a second excitation light source and collecting a second emission signal from the extended primer polynucleotide/taiget polynucleotide complex with a second emission filter;
Note[1]:
a. one of the first excitation light source and the second excitation light source has a wavelength of about 350 nm to about 410 nm, and the other one of the first excitation light source and the second excitation light source has a wavelength of about 450 nm to about 460 nm;
b. one of the first emission filter and the second emission filter has a detection wavelength of about 415 nm to about 450 nm, and the other one of the first emission filter and the second emission filter has a detection wavelength of about 480 nm to about 525 nm.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

246.59

Formula

C10H5ClF2O3

CAS 号
性状

固体

颜色

White to off-white

Emission (Em)

451

Excitation (Ex)

405

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
细胞实验: 

DMSO 中的溶解度 : 125 mg/mL (506.91 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.0553 mL 20.2766 mL 40.5531 mL
5 mM 0.8111 mL 4.0553 mL 8.1106 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.0553 mL 20.2766 mL 40.5531 mL 101.3829 mL
5 mM 0.8111 mL 4.0553 mL 8.1106 mL 20.2766 mL
10 mM 0.4055 mL 2.0277 mL 4.0553 mL 10.1383 mL
15 mM 0.2704 mL 1.3518 mL 2.7035 mL 6.7589 mL
20 mM 0.2028 mL 1.0138 mL 2.0277 mL 5.0691 mL
25 mM 0.1622 mL 0.8111 mL 1.6221 mL 4.0553 mL
30 mM 0.1352 mL 0.6759 mL 1.3518 mL 3.3794 mL
40 mM 0.1014 mL 0.5069 mL 1.0138 mL 2.5346 mL
50 mM 0.0811 mL 0.4055 mL 0.8111 mL 2.0277 mL
60 mM 0.0676 mL 0.3379 mL 0.6759 mL 1.6897 mL
80 mM 0.0507 mL 0.2535 mL 0.5069 mL 1.2673 mL
100 mM 0.0406 mL 0.2028 mL 0.4055 mL 1.0138 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
CellTracker Blue CMF2HC Dye
目录号:
HY-D1571
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