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  4. Cy3 methyltetrazine

Cy3 methyltetrazine  (Synonyms: TZ-Cy3)

目录号: HY-151776 纯度: 97.36%
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Cy3 methyltetrazine (TZ-Cy3) 是一种具有甲基四嗪结构单元的点击化学试剂，其对环辛烯具有高度反应性。Cy3 methyltetrazine 也是一种四嗪修饰的荧光探针，可用于分析溶液和活细胞中的蛋白质磷酸化。

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Cy3 methyltetrazine

Cy3 methyltetrazine Chemical Structure

CAS No. : 2183473-57-4

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规格 价格 是否有货 数量
1 mg ￥1250
In-stock
5 mg ￥2600
In-stock
10 mg ￥3400
In-stock
25 mg ￥5100
In-stock
50 mg 现货 询价
100 mg 现货 询价
200 mg   询价  
500 mg   询价  

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Customer Review

Cy3 methyltetrazine 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Cy3 methyltetrazine (TZ-Cy3) is a click chemistry reagent with methyltetrazine building blocks that is highly reactive towards cyclooctene. Cy3 methyltetrazine is also a tetrazine-modified fluorescent probe that can be used to analyze protein phosphorylation in solution and living cells[1].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Confocal imaging of phosphorylation in cells:
1. Culture H1299 cells in a coverglass-bottom imaging dish for 24 h (take H1299 cells as an example).
2. Incubate H1299 cells with different concentrations of ATP-NB (a ATP analogue functionalized by norbornene, which can penetrate cells and efficiently phosphorylate proteins in living cells) for 1 h.
3. Add Cy3 methyltetrazine to label the phosphorylated protein with fluorescence.
4. Incubate with fresh medium for 1 h.
5. Wash the cells twice with PBS to release the free Cy3 methyltetrazine probe.
6.Incubate the cells with 4% paraformaldehyde for 15 min and with 0.1% Triton X-100 solution at room temperature for 10 min.
7. Wash cells 4 times with PBS.
8. Use a confocal microscopy imaging system to obtain the high-resolution images of cells (Ex=488 nm; Em=561 nm).

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cy3 methyltetrazine 相关抗体:
分子量

727.64

Formula

C40H46BF4N7O
CAS 号
性状

固体

颜色

Brown to reddish brown

Emission (Em)

570
Excitation (Ex)

550
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 62.5 mg/mL (85.89 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.3743 mL 6.8715 mL 13.7431 mL
5 mM 0.2749 mL 1.3743 mL 2.7486 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

Cy3 methyltetrazine 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3743 mL 6.8715 mL 13.7431 mL 34.3576 mL
5 mM 0.2749 mL 1.3743 mL 2.7486 mL 6.8715 mL
10 mM 0.1374 mL 0.6872 mL 1.3743 mL 3.4358 mL
15 mM 0.0916 mL 0.4581 mL 0.9162 mL 2.2905 mL
20 mM 0.0687 mL 0.3436 mL 0.6872 mL 1.7179 mL
25 mM 0.0550 mL 0.2749 mL 0.5497 mL 1.3743 mL
30 mM 0.0458 mL 0.2291 mL 0.4581 mL 1.1453 mL
40 mM 0.0344 mL 0.1718 mL 0.3436 mL 0.8589 mL
50 mM 0.0275 mL 0.1374 mL 0.2749 mL 0.6872 mL
60 mM 0.0229 mL 0.1145 mL 0.2291 mL 0.5726 mL
80 mM 0.0172 mL 0.0859 mL 0.1718 mL 0.4295 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cy3 methyltetrazine2183473-57-4TZ-Cy3Fluorescent Dyecyclooctenemethyltetrazinefluorescent probeprotein phosphorylationH1299 cellsInhibitorinhibitorinhibit

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