Cyanine 3.5 chloride  (Synonyms: Cy3.5 NHS ester chloride; Cy 3.5 chloride)

Cyanine 3.5 (Cy3.5 NHS ester) chloride is an analog of Cy3.5 fluorophore. Cyanine 3.5 chloride is a reactive, red fluorescent dye. Cyanine 3.5 chloride is used for labeling of amino-groups in peptides, proteins, and oligonucleotides. (λ_ex=591 nm, λ_em=604 nm)^[1][2].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Cells: 1. Culture HepG2 cells in DMEM/HIGH GLUCOSE medium (containing 10% FBS, 100 U/mL penicillin G and 100 μg/mL streptomycin sulfate) at 37^oC in 5% CO₂ atmosphere. 2. Add 1×10⁶ HepG2 cells with logarithmic half-life in a confocal culture dish. Incubate cells at 37^oC, 5% CO₂, for 36 h. 3. Add 200 μL of 10 μg/mL Hoechst fluorescent dye 33342. Incubate cells at 37^o, 5% CO₂, for 20 minutes. 4. Changed the medium with 200 μL of Cy3.5-loaded FA-PEG-RH nanomicelles. 5. Take cell photos with a fluorescence microscope under 350 nm and 550 nm fluorescence excitation after 1h, 4 h, 8 h, and 16 h, respectively.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

690.27

C₄₂H₄₄ClN₃O₄

2231670-86-1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ning ZJ, et, al. Synthesis and evaluation of targeted nanomicelle delivery system with rhein as the hydrophobic end and its synergistic therapy effect on tumor. 2021 Dec;66.

  [2]. Kravchuk OI, et, al. Characterization of the 20S proteasome of the lepidopteran, Spodoptera frugiperda. Biochim Biophys Acta Proteins Proteom. 2019 Sep;1867(9):840-853.  [Content Brief]