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Cyanine3 maleimide tetrafluoroborate 

目录号: HY-D1618
产品使用指南

Cyanine3 maleimide tetrafluoroborate 是一种巯基特异性标记试剂,λex 为 550 nm,λem 为 580 nm。Cyanine3 maleimide tetrafluoroborate 可用于蛋白质和肽标记。

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Cyanine3 maleimide tetrafluoroborate Chemical Structure

Cyanine3 maleimide tetrafluoroborate Chemical Structure

CAS No. : 2755154-93-7

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Cyanine3 maleimide tetrafluoroborate is a specific labeling reagent for sulfhydryl groups with the λex of 550 nm and λem of 580 nm. Cyanine3 maleimide tetrafluoroborate can be used for protein and peptide labeling[1][2].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Protein: 1.The protein concentration was adjusted to 1 mg/mL for 100 μL. 2. A 100 μL aliquot of the protein solution was mixed with Cyanine3 maleimide tetrafluoroborate (8 μg) in DMSO (2 μL). 3. Incubated overnight at 4 °C in the dark. 4. Labeled proteins were desalted using a G25 desalting column to remove excessive Cyanine3 maleimide tetrafluoroborate. 5. Fluorescence intensities (FI) of labeled proteins were measured at excitation and emission wavelengths of 550 nm and 570 nm, respectively. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

666.56

Formula

C36H43BF4N4O3

CAS 号
Emission (Em)

570

Excitation (Ex)

550

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Cyanine3 maleimide tetrafluoroborate
目录号:
HY-D1618
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