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DFHO 

目录号: HY-136277
产品使用指南

DFHO 是 Corn 荧光适体的荧光配体。RNA 适体, Corn 结合 DFHO 的 Kd 值为 70 nM,并将其转化为荧光形式,从而使 RNA 在细胞中成像。

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DFHO Chemical Structure

DFHO Chemical Structure

CAS No. : 1420815-34-4

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Description

DFHO is a fluorogenic ligand of Corn fluorogenic aptamer. The RNA aptamer, Corn binds DFHO with a Kd value of 70 nM and converts it to a fluorescent form, enabling RNA imaging in cells[1][2].

IC50 & Target

Kd: 70 nM (Corn binds DFHO)[1]

In Vitro

Corn is tested in E. coli. Minimal fluorescence that is seen in DFHO-treated cells (20 μM) transformed with the empty vector. However robust yellow fluorescence is seen in E. coli expressing Corn[1].
Corn-DFHO exhibits markedly enhanced photostability in vitro. The Corn-DFHO complexes are imaged in mammalian cells. Since the U6 promoter is one of the three major Pol III promoters, Corn from the U6 promoter is expressed and replaced the majority of the U6 sequence with Corn, leaving a minimal 5′ capping element leader sequence. The majority of the cells expressed diffuse yellow fluorescence, most prominently in the cytoplasm)[1].
DFHO exhibits low background fluorescence and cytotoxicity, making it a potentially suitable fluorophore for activation by RNA aptamers and for imaging experiments in living cells[2].

Molecular Weight

281.21

Formula

C₁₂H₉F₂N₃O₃

CAS No.

1420815-34-4

SMILES

O=C(N1C)/C(N=C1/C=N/O)=C\C(C=C2F)=CC(F)=C2O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
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Keywords:

DFHOOthersCornyellowfluorescenceligandRNAdimerizationimagingliving cellsaptamerRFPInhibitorinhibitorinhibit

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产品名称:
DFHO
目录号:
HY-136277
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