Dornase alfa  (Synonyms: 阿法链道酶; rhDNase)

Dornase alfa (rhDNase) is a recombinant human deoxyribonuclease I (rhDNase) that can specifically degrade extracellular DNA. Dornase alfa catalyzes the cleavage of DNA released by neutrophils in respiratory mucus, reduces sputum viscosity, thereby improving mucus clearance efficiency, reducing airway obstruction and alleviating inflammatory responses. Dornase alfa can be used to improve lung function (such as FEV_1) in cystic fibrosis (CF), reduce the risk of acute pulmonary exacerbations, and has good in vivo tolerability. Dornase alfa acts locally on the respiratory tract through aerosol inhalation, specifically improving the high viscosity of mucus caused by DNA accumulation and related respiratory symptoms^[1][2][3][4].

Dornase alfa (2.5 mg/2.5 mL；雾化处理) 在体外酶活性测定中显示，经不同类型雾化器 (喷射型、静态型、振动型) 处理后，酶活性无显著损失，蛋白质结构保持完整，未出现聚集或降解^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Dornase alfa (2.5 mg/2.5 mL；雾化吸入；单剂量) 在小鼠模型中，经不同雾化器 (喷射型PARI BOY SX-red/blue喷嘴、静态mesh NE-U150、振动mesh NE-SM1) 雾化后，肺部递送的药物量以 PARI BOY SX-red 喷嘴最高，其气溶胶中位粒径 (Dv₅₀) 为 2.5-3.0 μm，且药物酶活性无显著损失，蛋白质结构保持完整^[3]。
Dornase alfa 可降低小鼠 B16 黑色素瘤模型的肿瘤转移数量，改变血清中的 cfDNA 模式^[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

143831-71-4

液体

Colorless to light yellow

阿法链道酶

1000 IU/mg protein

One Dnase unit results in an increase in absorbance at 260nm of 0.001/minute at 25°C when acting upon highly polymerized solution of DNA at pH-5.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Connie Yang, et al. Dornase alfa for cystic fibrosis. Cochrane Database Syst Rev. 2021 Mar 18;3(3):CD001127.  [Content Brief]

  [2]. Konstan MW, et al. Effect of dornase alfa on inflammation and lung function: potential role in the early treatment of cystic fibrosis. J Cyst Fibros. 2012 Mar;11(2):78-83.  [Content Brief]

  [3]. Chang KH, et al. Aerosol Delivery of Dornase Alfa Generated by Jet and Mesh Nebulizers. Pharmaceutics. 2020 Jul 31;12(8):721.  [Content Brief]

  [4]. Alekseeva L A, et al. Dornase alfa reduces the number of metastases in the B16 melanoma model and alters the pattern of cfDNA in the blood serum[J]. 2022.