1. Metabolic Enzyme/Protease
  2. Ser/Thr Protease
  3. Endoproteinase Glu-C

Endoproteinase Glu-C  (Synonyms: V8 protease; Glu-C)

目录号: HY-E70194
COA 产品使用指南

Endoproteinase GluC (V8 protease) 是一种丝氨酸蛋白酶。Endoproteinase GluC 能够水解一些丝氨酸蛋白酶抑制剂和所有类别的哺乳动物免疫球蛋白。

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Endoproteinase Glu-C Chemical Structure

Endoproteinase Glu-C Chemical Structure

CAS No. : 137010-42-5

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规格 价格 是否有货 数量
100 μg ¥6000
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Other Forms of Endoproteinase Glu-C:

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Endoproteinase GluC (V8 protease) is a serine proteinase. Endoproteinase GluC is able to hydrolyze some serpins and all classes of mammalian immunoglobulins[1].

体外研究
(In Vitro)

本产品可用于:蛋白质酶切测序分析、肽图谱分析和肽质量指纹图谱分析。
反应条件
反应 pH:4.0-10.0 之间均有活性
最适 pH:8.0-8.5
抑制剂:二异丙基氟磷酸(DFP)、α2-巨球蛋白和 Nα-P-甲苯磺酰基-L-赖氨酸氯甲基酮(TLCK)
分子量:24.0±2.4 kDa(SDS-PAGE)
使用方法
1)溶解:用酶切 buffer(25-50 mM NH4HCO3 pH 7.8)溶解目的蛋白。若溶解度不好,可对目的蛋白变性(添加尿素、SDS、DTT 或加热)。
2)酶切:V8 蛋白酶和目的蛋白推荐比例为 1/20-1/100(W/W),25℃ 或 37℃ 酶切 2-18 h。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

CAS 号
性状

油状物

颜色

Colorless to light yellow

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献

Endoproteinase Glu-C 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Endoproteinase Glu-C
目录号:
HY-E70194
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