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  3. Flubida-2

Flubida-2 是一种可渗透细胞的染料,可被细胞内的内酯酶水解为 Fubi-2 (水解后,Ex=492 nm, Em=517 nm)。通过将探针引导至亲和素融合蛋白所在的位置,Flubida-2 可用于检测细胞器中特定位点的 pH 值。

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Flubida-2 Chemical Structure

Flubida-2 Chemical Structure

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Customer Review

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Flubida-2 is a cell permeable dye which can be hydrolyzed to Fubi-2 by endoesterases in cells (after hydrolysis, Ex=492 nm, Em=517 nm). Flubida-2 can be used to detect pH at a specific site in a cell organelle by directing the probe to where avidin fusion proteins are located[1].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Dissolve Flubida-2 in DMSO (approximately 2 mM).
2. Mix the stock solution 1: 1 with 20% (w/v in DMSO) Pluronic F-127 (Molecular Probes), and dilute to the desired final concentration (2-4 μM) with serum-free DMEM.
3. 30 to 48 h post-transfection with either AV-KDEL or ST-AV DNA, HeLa cells are rinsed once with serum-free DMEM and loaded with 2-4 μM Flubida-2 for 3-5 h (or overnight for 10-15 h).
4. Chase the labeled cells with normal growth medium for at least 2 h, to allow excess dye-biotin to exit from the cytosol.
5. The strong avidin-biotin interaction ensures stable, specific avidin-Flubi- binding that resisted washing. Biotin starvation of the cells is not necessary before Flubida- loading, as staining was bright and stable.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

812.93

Formula

C86H96N8O20S2

Emission (Em)

517

Excitation (Ex)

492

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Flubida-2
目录号:
HY-D1688
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