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  4. Formamide (deionized)

Formamide (deionized)  (Synonyms: 去离子甲酰胺; Methanamide (deionized); Formimidic acid (deionized))

目录号: HY-Y0842B 纯度: 99.95%
COA 产品使用指南 技术支持

Formamide deionized (Methanamide deionizde) 是一种有效的 DNA 变性剂，可显著降低 DNA 在缓冲液中的稳定性。Formamide deionized 可被用作大鼠心脏细胞的脱管剂。此外，Formamide deionized 被广泛用作离子化合物、树脂和增塑剂的溶剂或化学原料。

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Formamide (deionized) Chemical Structure

Formamide (deionized) Chemical Structure

CAS No. : 75-12-7

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥440
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100 mL ￥400
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500 mL ￥1520
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5L   询价  

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Other Forms of Formamide (deionized):

Formamide (deionized) 相关抗体

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MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Formamide deionized (Methanamide deionizde) is an effective DNA denaturant that significantly reduces the stability of DNA in buffer solutions. Formamide deionized can also be used as a decalcifying agent for rat cardiac cells. Additionally, Formamide deionized is widely used as a solvent or chemical raw material for ion compounds, resins, and plasticizers[1][2][3][4][5][6][7].

体外研究
(In Vitro)

Formamide deionized (15 mol/L，15 分钟) 可诱导大鼠心肌细胞中 T 小管丢失[4]。
Formamide deionized (25 mL，10分钟) 诱导凋亡诱导剂 (Staurosporine (HY-15141) 处理的乳腺癌 MDA-468 细胞、白血病 MOLT-4 细胞及耐药亚系 MOLT-4/R ) 的 DNA 变性，但对非凋亡细胞 (活跃增殖的 MDA-468 和 MOLT-4 细胞) 的 DNA 没有影响[5]。
Formamide deionized (0.31-0.5 M，3 小时) 可抑制鸡胚胎神经上皮细胞的细胞间期核迁移并减少细胞中微管数量[6]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Formamide (deionized) 相关抗体:

Cell Migration Assay [6]

Cell Line: Neuroepithelial cells in the chick embryo
Concentration: 0.1-0.5 M
Incubation Time: 3 h
Result: Only affected mitosis which was blocked in metaphase so that mitotic figures accumulated near the neuro-coele (0.25 M).
Totally inhibited interkinetic nuclear migration and affected only slightly the cell asymmetry (0.31 M).
Considerably reduced the amount of cytoplasmic microtubules and that the cells became round (0.37 M).
Caused all microtubules had disappeared and all cells were spherical (0.5 M).
体内研究
(In Vivo)

Formamide deionized (80-160 mg/kg, 口服, 1 天 1 次，1 周 5 天，持续 2 周、3 个月或 2 年) 对雄性 B6C3F1 小鼠具有致癌活性[7]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: B6C3F1 mice[7]
Dosage: 10-160 mg/kg
Administration: Oral Gavage (p.o.), once daily, 5 days a week for 2 weeks, 3 months, or 2 years
Result: Resulted in a significant increase in the incidence of liver hemangiosarcoma, particularly in the 40 mg/kg and 80 mg/kg groups.
分子量

45.04

同用名

去离子甲酰胺; Methanamide (deionized); Formimidic acid (deionized)

Formula

CH3NO
CAS 号
性状

液体（密度：1.1334 g/cm³）

颜色

Colorless to light yellow

中文名称

去离子甲酰胺； 去离子氨基甲醛
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture and light
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 175 mg/mL (3885.44 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.95%

COA (282 KB) HNMR (230 KB) GC (171 KB)

产品使用指南 (1538 KB)
参考文献

Formamide (deionized) 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 22.2025 mL 111.0124 mL 222.0249 mL 555.0622 mL
5 mM 4.4405 mL 22.2025 mL 44.4050 mL 111.0124 mL
10 mM 2.2202 mL 11.1012 mL 22.2025 mL 55.5062 mL
15 mM 1.4802 mL 7.4008 mL 14.8017 mL 37.0041 mL
20 mM 1.1101 mL 5.5506 mL 11.1012 mL 27.7531 mL
25 mM 0.8881 mL 4.4405 mL 8.8810 mL 22.2025 mL
30 mM 0.7401 mL 3.7004 mL 7.4008 mL 18.5021 mL
40 mM 0.5551 mL 2.7753 mL 5.5506 mL 13.8766 mL
50 mM 0.4440 mL 2.2202 mL 4.4405 mL 11.1012 mL
60 mM 0.3700 mL 1.8502 mL 3.7004 mL 9.2510 mL
80 mM 0.2775 mL 1.3877 mL 2.7753 mL 6.9383 mL
100 mM 0.2220 mL 1.1101 mL 2.2202 mL 5.5506 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Formamide (deionized)75-12-7Methanamide (deionized)Formimidic acid (deionized)去离子甲酰胺 去离子氨基甲醛Biochemical Assay ReagentsDenaturing Polyacrylamide GelsNucleic Acid HybridizationInhibitorinhibitorinhibit

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