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  2. Fluorescent Dye
  3. Fura Red

Fura Red 是一种 Ca2+ 敏感型荧光染料,荧光随 [Ca2+] 浓度升高而降低。

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Fura Red Chemical Structure

Fura Red Chemical Structure

CAS No. : 149732-62-7

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  • 生物活性

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生物活性

Fura Red is a Ca2+-sensitive fluorescent dye, which decreases in fluorescence with rising [Ca2+][1].

体外研究
(In Vitro)

Guidelines[2] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Cells:
1. Incubate the cells according to your normal protocol.
2. Suspend cells at 1x107 cells/mL in 37°C Hanks Balanced Salt Solution (HBSS) with 1 μM Fura Red, AM unless specified otherwise and 0.01% Pluronic F127, and incubate in a 37°C water bath for 30 minutes.
3. Wash cells in HEPES Buffered Saline Solution (HBSS with 1 mM CaCl2, 0.5 mM MgCl2, 0.1% BSA, 10 mM HEPES) and resuspend to 1x107 cells/mL in HEPES Buffered Saline Solution with viability dye, SYTOX Green.
4. Equilibrate cells for ≥10 minutes in a 37°C water bath.
5. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader. Ratiometric analysis of Fura Red is measured by excitation by the Violet laser (406 nm) and the Green laser (532 nm). Emission is detected by two different filter sets: increases in emission are monitored off the Violet laser (630LP and 660/20 BP), while a decrease in emission are detected off the Green laser (685LP and 710/50 BP).

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

944.87

Formula

C41H44N4O20S

CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献

Fura Red 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Fura Red
目录号:
HY-104056
需求量: