Gol-NTR 

Gol-NTR is a Golgi-targetable probe with high selectivity and sensitivity. Gol-NTR is Nitroreductase (NTR)-activated and has visualization acute lung injury (ALI) and repair function. Gol-NTR has a low detection limit of 54.8 ng/mL. Gol-NTR can be used for the research for monitoring and assessing research response of sepsis-induced ALI^[1].

Gol-NTR 对 NTR 的荧光标记^[1]
(1) 用 DMSO 溶液配制 1.0 mM Gol-NTR 原液。
(2) 用 DMSO 溶液稀释原液配制 5.0 μM Gol-NTR 工作液。
(3) 5.0 μM Gol-NTR 与 50 μM NADH 混合在含 5% DMSO 的 PBS 缓冲液 (10 mM，pH 7.4) 中，然后加入适量的 NTR。
(4) 37℃ 孵育 30 min 后，在 405 nm (狭缝宽度：d_ex/d_em=5/5 nm) 下记录光谱。
Gol-NTR 对 A549 细胞中 NTR 的荧光标记^[1]
(1) A549 细胞在不同氧浓度 (1%、5%、10%、15% 和 20% O₂) 下培养 8 h。
(2) 用磷酸盐缓冲盐水 (PBS) 冲洗 A549 细胞。
(3) 5.0 μM Gol-NTR 处理 A549 细胞 1 h。
(4) 用共聚焦荧光成像观察 A549 细胞的荧光图像。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内成像^[1]
(1) C57BL/6 雄性小鼠 (6-8 周龄，体重 20-22 g) 预注射 300 μL DMOG (25 mg/mL)，24 h 后，腹腔注射 300 μL LPS (10 mg/kg)，持续 6 h。
(2) 颈椎脱臼处死小鼠，收集肺器官。
(3) PBS 冲洗后，用 50 μM Gol-NTR 在 PBS 中孵育 1 h。
(4) PBS 冲洗后，在小型动物成像系统上进行荧光成像 (420 nm 的激发波长和 510 nm 的发射波长)。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

467.40

C₂₄H₁₆F₃N₃O₄

730

680

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Tang Z, et al. Precise Monitoring and Assessing Treatment Response of Sepsis-Induced Acute Lung Hypoxia with a Nitroreductase-Activated Golgi-Targetable Fluorescent Probe. Anal Chem. 2022 Oct 25;94(42):14778-14784.  [Content Brief]