2. MCE Kits
  3. Protein Biology
  4. Protein Electrophoresis & WB
  5. Transfer Buffer
  6. Tris-Glycine Powder (1 L of 1×)

Tris-Glycine Powder (1 L of 1×) 

Tris-甘氨酸速溶颗粒 (1 L of 1×)

Cat. No.: HY-K1021
Manual SDS

MCE Tris-Glycine Powder (1 L of 1×) 主要成分为三羟甲基氨基甲烷（Tris）和甘氨酸（Glycine），常用作非变性蛋白聚丙烯酰胺凝胶电泳（Native-PAGE）缓冲液。

Tris-Glycine Powder (1 L of 1×)

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
Free Sample (2 pouches)   Apply now  
10 pouches ￥175 In-stock
50 pouches ￥520 In-stock

* Please select Quantity before adding items.

  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

Tris-Glycine (TG) is widely used as an electrophoresis buffer for Native-PAGE. TG buffer is also used for Western Blot when prepared in 20% methanol.

MCE Tris-Glycine Powder (1 L of 1×) consists of Tris and Glycine. Each pouch can be diluted to 1 L 1× TG buffer, in which the concentration of Tris is 25 mM and the concentration of Glycine is 192 mM. This product provides a convenient way to make TG buffer and eliminates the need to weigh and mix individual components.

Storage

Store at room temperature for 3 years.

Protocol

1.   Add 1 pouch of Tris-Glycine Powder into the cleaned beaker, dissolve with 600 mL distilled water (or 200 mL methanol and 400 mL distilled water when used for membrane transfer in Western Blot) under a magnetic stirrer.

2.    Add distilled water to the solution in step 1 until the total volume is 1 L. The final solution is 1× TG buffer.

Note: The pH of the 1× TG buffer is 8.3 ± 0.2 @ 25°C.

Components
Components HY-K1021-10 pouches HY-K1021-50 pouches
Tris-Glycine Powder (1 L of 1×) 10 pouches 5 × 10 pouches
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称：
Tris-甘氨酸速溶颗粒 (1 L of 1×)
目录号：
HY-K1021
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