2. Academic Validation
  3. N-Salicyloyltryptamine, an N-Benzoyltryptamine Analogue, Induces Vasorelaxation through Activation of the NO/sGC Pathway and Reduction of Calcium Influx

N-Salicyloyltryptamine, an N-Benzoyltryptamine Analogue, Induces Vasorelaxation through Activation of the NO/sGC Pathway and Reduction of Calcium Influx

  • Molecules. 2018 Jan 28;23(2):253. doi: 10.3390/molecules23020253.
Robson Cavalcante Veras 1 2,  Darizy Flávia Silva 3,  Lorena Soares Bezerra 4,  Valéria Lopes de Assis 5,  Walma Pereira de Vasconcelos 6,  Maria do Carmo Alustau 7,  José George Ferreira de Albuquerque 8,  Fabíola Fialho Furtado 9,  Islania Giselia de Albuquerque Araújo 10,  Fátima de Lourdes Assunção Araújo de Azevedo 11,  Thais Porto Ribeiro 12,  José Maria Barbosa-Filho 13 14,  Stanley Juan Chavez Gutierrez 15,  Isac Almeida Medeiros 16 17
Affiliations

Affiliations

  • 1 Department of Pharmaceutical Sciences, Federal University of Paraíba (UFPB), João Pessoa 58059-900, Brazil. robsonveras@ccs.ufpb.br.
  • 2 Postgraduate Program of Nutrition Science/CCS/Federal University of Paraíba (UFPB). robsonveras@ccs.ufpb.br.
  • 3 Department of Biorregulation, Federal University of Bahia (UFBA), Av. Reitor Miguel Calmon, S/N, Vale do Canela, Salvador 40110-902, Brazil. darizy.silva@ufba.br.
  • 4 Postgraduate Program of Nutrition Science/CCS/Federal University of Paraíba (UFPB). lorena.sbezerra@gmail.com.
  • 5 Postgraduate Program of Natural Products and Bioactive Synthetics/CCS/Universidade Federal da Paraíba (UFPB), João Pessoa 58059-900, Brazil. islania.ltf@gmail.com.
  • 6 Postgraduate Program of Natural Products and Bioactive Synthetics/CCS/Universidade Federal da Paraíba (UFPB), João Pessoa 58059-900, Brazil. jbarbosa@ltf.ufpb.br.
  • 7 Postgraduate Program of Natural Products and Bioactive Synthetics/CCS/Universidade Federal da Paraíba (UFPB), João Pessoa 58059-900, Brazil. isac@ltf.ufpb.br.
  • 8 Postgraduate Program of Natural Products and Bioactive Synthetics/CCS/Universidade Federal da Paraíba (UFPB), João Pessoa 58059-900, Brazil.
  • 9 Postgraduate Program of Natural Products and Bioactive Synthetics/CCS/Universidade Federal da Paraíba (UFPB), João Pessoa 58059-900, Brazil. fabiola.fialho@gmail.com.
  • 10 Department of Pharmaceutical Sciences, Federal University of Paraíba (UFPB), João Pessoa 58059-900, Brazil.
  • 11 Postgraduate Program of Natural Products and Bioactive Synthetics/CCS/Universidade Federal da Paraíba (UFPB), João Pessoa 58059-900, Brazil. val_farm@ltf.ufpb.com.
  • 12 Postgraduate Program of Natural Products and Bioactive Synthetics/CCS/Universidade Federal da Paraíba (UFPB), João Pessoa 58059-900, Brazil. thaispribeiro@hotmail.com.
  • 13 Department of Pharmaceutical Sciences, Federal University of Paraíba (UFPB), João Pessoa 58059-900, Brazil. walmasjp@hotmail.com.
  • 14 Postgraduate Program of Natural Products and Bioactive Synthetics/CCS/Universidade Federal da Paraíba (UFPB), João Pessoa 58059-900, Brazil. walmasjp@hotmail.com.
  • 15 Postgraduate Program of Natural Products and Bioactive Synthetics/CCS/Universidade Federal da Paraíba (UFPB), João Pessoa 58059-900, Brazil. stanleychavez@ufpi.edu.br.
  • 16 Department of Pharmaceutical Sciences, Federal University of Paraíba (UFPB), João Pessoa 58059-900, Brazil. maria.alustau@ufcg.edu.br.
  • 17 Postgraduate Program of Natural Products and Bioactive Synthetics/CCS/Universidade Federal da Paraíba (UFPB), João Pessoa 58059-900, Brazil. maria.alustau@ufcg.edu.br.
PMID: 29382081 DOI: 10.3390/molecules23020253
Abstract

Benzoyltryptamine analogues act as neuroprotective and spasmolytic agents on smooth muscles. In this study, we investigated the ability of N-salicyloyltryptamine (STP) to produce vasorelaxation and determined its underlying mechanisms of action. Isolated rat mesenteric arteries with and without functional endothelium were studied in an isometric contraction system in the presence or absence of pharmacological inhibitors. Amperometric experiments were used to measure the nitric oxide (NO) levels in CD31+ cells using flow cytometry. GH3 cells were used to measure Ca2+ currents using the whole cell patch clamp technique. STP caused endothelium-dependent and -independent relaxation in mesenteric rings. The endothelial-dependent relaxations in response to STP were markedly reduced by L-NAME (endothelial NO synthase-eNOS-inhibitor), jHydroxocobalamin (NO scavenger, 30 µM) and ODQ (soluble Guanylyl Cyclase-sGC-inhibitor, 10 µM), but were not affected by the inhibition of the formation of vasoactive prostanoids. These results were reinforced by the increased NO levels observed in the amperometric experiments with freshly dispersed CD31+ cells. The endothelium-independent effect appeared to involve the inhibition of voltage-gated Ca2+ channels, due to the inhibition of the concentration-response Ca2+ curves in depolarizing solution, the increased relaxation in rings that were pre-incubated with high extracellular KCl (80 mM), and the inhibition of macroscopic Ca2+ currents. The present findings show that the activation of the NO/sGC/cGMP pathway and the inhibition of gated-voltage Ca2+ channels are the mechanisms underlying the effect of STP on mesenteric arteries.

Keywords

calcium; endothelium; mesenteric; nitric oxide; rats; vascular.

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