2. Academic Validation
  3. UPLC-ESI-TOF-MS Profiling of Metabolome Alterations in Barley (Hordeum vulgare L.) Leaves Induced by Bipolaris sorokiniana

UPLC-ESI-TOF-MS Profiling of Metabolome Alterations in Barley (Hordeum vulgare L.) Leaves Induced by Bipolaris sorokiniana

  • J Agric Food Chem. 2025 Oct 1;73(39):24662-24687. doi: 10.1021/acs.jafc.5c05419.
Lisa Kurzweil 1,  Timo D Stark 2,  Karina Hille 2,  Felix Hoheneder 3,  Jana Mrtva 2,  Johann Hausladen 4,  Miriam Lenk 5,  Mohammed Saddik Motawia 6,  Nicole Strittmatter 7,  A Corina Vlot 5 8,  Klaus Pillen 9,  Mette So̷rensen 6 10,  Birger L Mo̷ller 6,  Ralph Hückelhoven 3,  Corinna Dawid 1 2 11
Affiliations

Affiliations

  • 1 Professorship for Functional Phytometabolomics, TUM School of Life Sciences, Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany.
  • 2 Chair of Food Chemistry and Molecular Sensory Science, TUM School of Life Sciences, Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany.
  • 3 Chair of Phytopathology, TUM School of Life Sciences, Technical University of Munich, Emil-Ramann-Str. 2, 85354 Freising, Germany.
  • 4 Plant Technology Center, TUM School of Life Sciences, Technical University of Munich, Dürnast 9, 85354 Freising, Germany.
  • 5 Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany.
  • 6 Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiskberg C, Copenhagen Denmark.
  • 7 Professorship for Analytical Chemistry, Technical University of Munich, Lichtenbergstr. 4, 85748 Garching, Germany.
  • 8 Chair of Crop Plant Genetics, Faculty of Life Sciences: Food, Nutrition and Health, University of Bayreuth, Fritz-Hornschuch-Straße 13, 95326 Kulmbach, Germany.
  • 9 Chair of Plant Breeding, Martin-Luther-University Halle-Wittenberg, Betty-Heimann-Str. 3, 06120 Halle (Saale), Germany.
  • 10 Novo Nordisk Pharmatech, Ko̷benhavnsvej 216, 4600 Ko̷ge, Copenhagen Denmark.
  • 11 Leibniz Institute for Food Systems Biology at the Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany.
PMID: 40963221 DOI: 10.1021/acs.jafc.5c05419
Abstract

Spot blotch of barley (Hordeum vulgare L.), caused by Bipolaris sorokiniana, is responsible for major losses in crop yield. Breeding-resistant barley varieties have proven to be an effective countermeasure for protecting agricultural production. Plants react to pathogen attacks by up-regulating secondary metabolites. Marker compounds for a B. sorokiniana Infection are examined by untargeted UPLC-TOF-MS metabolomics and lipidomics techniques. Through the analysis of nine quantitatively resistant and susceptible barley genotypes, derived from the nested association mapping population HEB-25, followed by structure identification experiments and spore germination assays, 57 metabolites are identified. In addition to previously known metabolites, the unknown compounds 5-carboxydidehydroblumenol C-9-O-ß-d-glucoside (46) and grasshopper ketone 3-sulfate (47) were elucidated. 5-Carboxyblumenol C-9-O-ß-d-glucoside (45) was described for the first time in barley leaves. Pheophytin derivatives, oxylipins, linolenate-conjugated lipids, and flavone glycosides were described for the first time in connection with infections by phytopathogenic fungi or resistance in barley.

Keywords

Bipolaris sorokiniana; MS imaging; barley; lipids; metabolomics; untargeted LC−MS.

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