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  4. Nile Red (solution)

Nile Red (solution)  (Synonyms: Nile Blue A oxazone (solution); Phenoxazone 9 (solution))

目录号: HY-D0718Y
产品使用指南 技术支持

Nile Red (solution) (Nile Blue A oxazone (solution)) 是一种亲脂染色。尼罗红具有环境敏感性荧光。尼罗红在富脂环境中荧光强烈，而在水介质中荧光微弱。尼罗红是荧光显微镜和流式细胞荧光法检测细胞内脂滴的极好的重要染色剂。尼罗红将细胞内脂滴染成红色。荧光波长559/635 nm。

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Nile Red (solution)

Nile Red (solution) Chemical Structure

CAS No. : 7385-67-3

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规格 价格 是否有货
31.83 μg (1 mM * 100 μL in DMSO) ￥212
询问价格 & 货期
63.67 μg (1 mM * 200 μL in DMSO) ￥298
询问价格 & 货期
159.18 μg (1 mM * 500 μL in DMSO) ￥536
询问价格 & 货期
318.37 μg (1 mM * 1 mL in DMSO) ￥731
询问价格 & 货期

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Nile Red (solution) 的其他形式现货产品:

Customer Review

Other Forms of Nile Red (solution):

Nile Red (solution) 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 85 篇科研文献

IF

    Nile Red (solution) purchased from MCE. Usage Cited in: Nat Commun. 2022 Oct 5;13(1):5871.  [Abstract]

    Isolated oocytes are fixed with 4% paraformaldehyde for 1 hour at room temperature and washed 3 times with 1% BSA in PBS. Then, they are stained with 10 µg/ml Nile red at 4 °C overnight.

    Nile Red (solution) purchased from MCE. Usage Cited in: Nucleic Acids Res. 2022 Jun 24;50(12):6953-6967.  [Abstract]

    The wild-type or G4-mutant human hepatic adenocarcinoma HepG2 cells are stained with Nile Red reagent to visualize the lipids with an Olympus Fluoview FV1000 confocal microscope.

    Nile Red (solution) purchased from MCE. Usage Cited in: Nano Today. 47 (2022) 101675

    Subcellular biodistribution of NileRed-labeled PFD Liposome nano-particles in 4T1 and BT-549 cells are visualized by confocal laser microscopy. Blue, DAPI; Green, WGA 488; Red, NileRed-labeled PFD Liposome.

    Nile Red (solution) purchased from MCE. Usage Cited in: EMBO J. 2022 Aug 16;41(16):e110439.  [Abstract]

    Representative pictures of Nile red-stained human BAs differentiated from hESC-derived brown progenitors.

    Nile Red (solution) purchased from MCE. Usage Cited in: J Transl Med. 2022 May 14;20(1):222.  [Abstract]

    Living cells are incubated with Nile Red (1 μM) for 15 min at 37 °C in dark. Then, cells are washed with HBSS/Ca/Mg and analyzed by fluorescence microscopy.

    Nile Red (solution) purchased from MCE. Usage Cited in: Eur J Pharmacol. 2023 Jan 5:938:175428.  [Abstract]

    Hepatic lipid and FFA content are determined by Nile red staining of liver frozen sections.

    Nile Red (solution) purchased from MCE. Usage Cited in: Theranostics. 2021 Jan 1;11(5):2149-2169.  [Abstract]

    Injected 5 μg of Nile Red lipid dye into the tail vein of mice to label triglycerides in the liver prior to intravital imaging.

    Nile Red (solution) purchased from MCE. Usage Cited in: Cell Prolif. 2021 Nov;54(11):e13134.  [Abstract]

    For lipid droplet staining, fixed cells are stained for 30 minutes with 0.1 µg/ml Nile Red (MCE), and the nucleus is counterstained using DAPI.

    Nile Red (solution) purchased from MCE. Usage Cited in: Int J Mol Sci. 2021 May 31;22(11):5951.  [Abstract]

    For lipid staining, fat bodies are submerged in Nile red solution at a final working concentration of 10 μg/mL in an acetone/water (1:9) mixture and visualized using a fluorescent microscope at Ex543/Em626 nm.

    Nile Red (solution) purchased from MCE. Usage Cited in: Nat Commun. 2020 Jan 13;11(1):240.   [Abstract]

    HSCs are stained with Nile Red reagents and Bodipy493/503 to visualize the lipids with a light microscope.

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Nile Red (solution) (Nile Blue A oxazone (solution)) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm[1].

    体外研究
    (In Vitro)

    1.1 制备储存液
    用 DMSO 配制 1mM 储存液。
    1.2 工作液的配制
    用预热好的无血清细胞培养基或 PBS 稀释储存液，终浓度为 200-1000 nM。
    注：请根据实际情况调整 Nile Red 工作液浓度，且现用现配。
    2.细胞染色
    2.1 悬浮细胞：离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。
    贴壁细胞：弃去培养基，加入胰蛋白酶消化细胞。离心弃去上清后，加入 PBS 洗涤两次，每次 5 分钟。
    2.2 加入 1 mL Nile Red 工作液，室温孵育 5-10 分钟。
    2.3 400 g，4℃ 离心 3-4 分钟，弃去上清。
    2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。
    2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，使用荧光显微镜进行观察。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Nile Red (solution) 相关抗体:
    体内研究
    (In Vivo)

    当用尼罗红染色的秀丽隐杆线虫发出绿色荧光时，可以在整个肠道和其他组织中观察到离散的脂质体，它们或成簇或均匀分散，具体取决于动物的基因型或实验治疗[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    318.37

    Formula

    C20H18N2O2
    CAS 号
    中文名称

    尼罗红 （溶液）
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    Please store the product under the recommended conditions in the Certificate of Analysis.
    纯度 & 产品资料
    参考文献

    Nile Red (solution) 相关分类

    • 摩尔计算器

    • 稀释计算器

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量   浓度   体积   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Nile Red (solution)7385-67-3Nile Blue A oxazone (solution)Phenoxazone 9 (solution)尼罗红 （溶液）Fluorescent DyeBenzophenoxazonehydrophobiclipiddropletsfluorescentneutrallysochromeInhibitorinhibitorinhibit

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