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  2. PIPES

PIPES (Synonyms: 1,4-Piperazinediethanesulfonic acid)

目录号: HY-D0875
产品使用指南

PIPES (1,4-Piperazinediethanesulfonic acid) 是 PIPES 缓冲液的重要组成部分,常用作生化试剂。

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PIPES Chemical Structure

PIPES Chemical Structure

CAS No. : 5625-37-6

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Size Price Stock Quantity
Free Sample (0.1-0.5 mg)   Apply now  
10 mM * 1 mL in Water ¥550 In-stock
500 mg ¥500 In-stock
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Description

PIPES (1,4-Piperazinediethanesulfonic acid) is an important component of PIPES buffer agent used in biochemistry[1].

In Vitro

To prepare the pH PIPES buffer, 173 g of 1,4-piperazinediethanesulfonic acid are dissolved into 1 L of deionized water. The pH of the PIPES buffer is adjusted to 6.8 by adding pellets of sodium hydroxide.
PIPES buffer can be used to PIPES buffer, it can prevent the glutaraldehyde fixation induced lipid loss and artifacts[1].

Molecular Weight

302.37

Formula

C₈H₁₈N₂O₆S₂

CAS No.

5625-37-6

SMILES

O=S(CCN1CCN(CCS(=O)(O)=O)CC1)(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

H2O : 5 mg/mL (16.54 mM; ultrasonic and adjust pH to 7 with NaOH)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3072 mL 16.5360 mL 33.0721 mL
5 mM 0.6614 mL 3.3072 mL 6.6144 mL
10 mM 0.3307 mL 1.6536 mL 3.3072 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。 -80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

References
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

PIPES1,4-Piperazinediethanesulfonic acidOthersbuffer PIPEScell culturesample prepareglutaraldehyde fixativeInhibitorinhibitorinhibit

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产品名称:
PIPES
目录号:
HY-D0875
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