1. Metabolic Enzyme/Protease
  2. Ser/Thr Protease
  3. Recombinant Proteinase K

Recombinant Proteinase K  (Synonyms: 重组蛋白酶 K)

目录号: HY-P3150
COA 产品使用指南

Recombinant Proteinase K 是一种丝氨酸蛋白酶 (serine protease),它能裂解脂肪族和芳香族氨基酸的羧基端肽键。Recombinant Proteinase K 可用于消化蛋白质和清除核酸制剂中的污染。

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Recombinant Proteinase K Chemical Structure

Recombinant Proteinase K Chemical Structure

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规格 是否有货
50 mg
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100 mg
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Top Publications Citing Use of Products
  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Recombinant Proteinase K is a serine protease that cleaves the carboxy-terminated peptide bonds of aliphatic and aromatic amino acids. Recombinant Proteinase K can be used to digest proteins and remove contamination from nucleic acid preparations[1].

体外研究
(In Vitro)

Proteinase K digestion[1]
1. Cells are resuspended in lysis bufferand finally lysed on ice for 15 minutes.
2. The crude lysates are obtained by centrifugation at 4°C for 15 minutes. Proteinase K is added to a 20 µl aliquot to get the final concentrations of 2, 4 and 8 µg/ml. Reactions are carried out at 37°C for 30 minutes.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

性状

固体

颜色

Off-white to light yellow

中文名称

重组蛋白酶 K

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料

纯度: ≥95.0%

参考文献

Recombinant Proteinase K 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Recombinant Proteinase K
目录号:
HY-P3150
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