ACE2 蛋白, Mouse (HEK293, His-Fc)

ACE2 Protein, a crucial carboxypeptidase in the renin-angiotensin system, regulates blood volume and vascular resistance, maintaining cardiovascular homeostasis. It converts angiotensin I to anti-hypertrophic angiotensin 1-9 and angiotensin II to vasodilatory angiotensin 1-7, countering vasoconstriction. ACE2 also regulates amino acid transport, interacting with SLC6A19, and modulates vasoactive peptides like neurotensin and kinetensin. Its multifaceted functions make ACE2 pivotal in cardiovascular and amino acid homeostasis. ACE2 Protein, Mouse (HEK293, His-Fc) is the recombinant mouse-derived ACE2 protein, expressed by HEK293, with C-hFc, C-His, C-8*His labeled tag. The total length of ACE2 Protein, Mouse (HEK293, His-Fc) is 723 a.a., with molecular weight of ~130 kDa.

ACE2 是肾素-血管紧张素激素系统中一种重要的反调节羧肽酶，是血容量和全身血管阻力的关键调节因子，有助于心血管稳态。其酶活性包括将血管紧张素 I 转化为血管紧张素 1-9（一种在心肌细胞中具有抗肥大作用的肽），以及将血管紧张素 II 转化为血管紧张素 1-7（以其血管舒张和抗增殖特性而闻名），从而抵消血管紧张素的血管收缩作用。血管紧张素II。ACE2 进一步在氨基酸转运中发挥作用，作为氨基酸转运蛋白 SLC6A19 的结合伴侣，调节其细胞表面运输和活性。此外，ACE2 有助于调节各种血管活性肽，例如神经降压素、运动紧张素和去精氨酸缓激肽，以及其他生物肽，例如 apelins、酪啡肽和强啡肽 A。其多方面功能使 ACE2 成为心血管疾病的关键参与者。和氨基酸稳态。

Measured by its binding ability in a functional ELISA. Immobilized Mouse ACE2, at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S1 protein. The ED₅₀ for this effect is 149.1 ng/mL, corresponding to a specific activity is 6706.9 Unit/mg.

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  Measured by its binding ability in a functional ELISA. Immobilized Mouse ACE2, at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S1 protein. The ED₅₀ for this effect is 149.1 ng/mL, corresponding to a specific activity is 6706.9 Unit/mg.

Mouse

HEK293

C-hFc;C-His;C-8*His

Q8R0I0-1 (Q18-T740)

70008  [NCBI]

QSLTEENAKTFLNNFNQEAEDLSYQSSLASWNYNTNITEENAQKMSEAAAKWSAFYEEQSKTAQSFSLQEIQTPIIKRQLQALQQSGSSALSADKNKQLNTILNTMSTIYSTGKVCNPKNPQECLLLEPGLDEIMATSTDYNSRLWAWEGWRAEVGKQLRPLYEEYVVLKNEMARANNYNDYGDYWRGDYEAEGADGYNYNRNQLIEDVERTFAEIKPLYEHLHAYVRRKLMDTYPSYISPTGCLPAHLLGDMWGRFWTNLYPLTVPFAQKPNIDVTDAMMNQGWDAERIFQEAEKFFVSVGLPHMTQGFWANSMLTEPADGRKVVCHPTAWDLGHGDFRIKMCTKVTMDNFLTAHHEMGHIQYDMAYARQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLPSDFQEDSETEINFLLKQALTIVGTLPFTYMLEKWRWMVFRGEIPKEQWMKKWWEMKREIVGVVEPLPHDETYCDPASLFHVSNDYSFIRYYTRTIYQFQFQEALCQAAKYNGSLHKCDISNSTEAGQKLLKMLSLGNSEPWTKALENVVGARNMDVKPLLNYFQPLFDWLKEQNRNSFVGWNTEWSPYADQSIKVRISLKSALGANAYEWTNNEMFLFRSSVAYAMRKYFSIIKNQTVPFLEEDVRVSDLKPRVSFYFFVTSPQNVSDVIPRSEVEDAIRMSRGRINDVFGLNDNSLEFLGIHPTLEPPYQPPVT

Approximately 130 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 1 mM ZnCl₂, pH 7.5 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.