ACE2 蛋白, Rat (HEK293, His)

ACE2 is an important carboxypeptidase in the renin-angiotensin system that complexly regulates cardiovascular homeostasis. It specifically converts angiotensin I to angiotensin 1-9 and angiotensin II to angiotensin 1-7, exerting antihypertrophic and vasodilatory effects. ACE2 Protein, Rat (HEK293, His) is the recombinant rat-derived ACE2 protein, expressed by HEK293 , with C-His labeled tag.

ACE2 是肾素-血管紧张素激素系统中一种重要的反调节羧肽酶，是协调血容量、全身血管阻力和整体心血管稳态的关键调节因子。这种多功能酶在其作用中表现出显着的特异性，可将血管紧张素 I 转化为血管紧张素 1-9，将血管紧张素 II 转化为血管紧张素 1-7。这些生物活性肽在心肌细胞中表现出抗肥厚作用，并作为有益的血管扩张剂，抵消血管紧张素 II 的血管收缩特性。除了在血管紧张素系统中的作用外，ACE2 通过裂解各种血管活性肽（包括神经降压素、运动紧张素和去精氨酸缓激肽）而展现出多功能性。值得注意的是，ACE2 与氨基酸转运密切相关，作为肠道中氨基酸转运蛋白 SLC6A19 的结合伴侣。通过参与运输、表面表达和催化活性调节，ACE2 在氨基酸转运过程的调节中发挥着至关重要的作用。

Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH. The specific activity is 20028.43 pmol/min/µg, as measured under the described conditions.

Materials
Detection buffer: 50 mM Tris, 1M Nacl, pH 7.0
ACE2 Protein, Rat (HEK293, His) (HY-P72809)
Substrate: Mca-YVADAPK(Dnp) -OH was solubilized to 2 mM with DMSO
Standard: MCA-Pro-Leu-OH

Procedure
1. Dilute MCA-Pro-Leu-OH to 50, 25, 12.5, 6.25, 3.125, 1.5625, 0.78, 0.39, 0.2, 0 μmol/L with assay buffer, and then add 100 μL of each buffer into wells to start the reaction. The excitation and emission wavelengths were 320 nm and 405 nm , respectively, and read in kinetic mode for 5 min. The standard curve was made with the concentration of the standard as the horizontal coordinate and the measured RFU as the vertical coordinate, and the standard curve formula was obtained.
2. Dilute Rat ACE2 to 0.05, 0.5 , 5 μg/mL with assay buffer.
3. The substrate was diluted to 20 μM with assay buffer.
4. Add different concentrations of ACE2 to the black well plate at 50 μL per well and start the reaction by adding 50 μL of 20 μM substrate. Blank wells were added with 50 μL of assay buffer and 50 μL of 20 μM substrate.
5. Read in kinetic mode for 5 minutes at 320 nm and 405 nm excitation and emission wavelengths, respectively.
6. Calculate the specific activity:

Rat

HEK293

C-His

Q5EGZ1 (Q18-T740)

302668  [NCBI]

Extracellular Domain

QSLIEEKAESFLNKFNQEAEDLSYQSSLASWNYNTNITEENAQKMNEAAAKWSAFYEEQSKIAQNFSLQEIQNATIKRQLKALQQSGSSALSPDKNKQLNTILNTMSTIYSTGKVCNSMNPQECFLLEPGLDEIMATSTDYNRRLWAWEGWRAEVGKQLRPLYEEYVVLKNEMARANNYEDYGDYWRGDYEAEGVEGYNYNRNQLIEDVENTFKEIKPLYEQLHAYVRTKLMEVYPSYISPTGCLPAHLLGDMWGRFWTNLYPLTTPFLQKPNIDVTDAMVNQSWDAERIFKEAEKFFVSVGLPQMTPGFWTNSMLTEPGDDRKVVCHPTAWDLGHGDFRIKMCTKVTMDNFLTAHHEMGHIQYDMAYAKQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLPSNFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFQDKIPREQWTKKWWEMKREIVGVVEPLPHDETYCDPASLFHVSNDYSFIRYYTRTIYQFQFQEALCQAAKHDGPLHKCDISNSTEAGQKLLNMLSLGNSGPWTLALENVVGSRNMDVKPLLNYFQPLFVWLKEQNRNSTVGWSTDWSPYADQSIKVRISLKSALGKNAYEWTDNEMYLFRSSVAYAMREYFSREKNQTVPFGEADVWVSDLKPRVSFNFFVTSPKNVSDIIPRSEVEEAIRMSRGRINDIFGLNDNSLEFLGIYPTLKPPYEPPVT

105-120 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.