ACKR3 蛋白, Human (His-SUMO)

ACKR3 is an atypical chemokine receptor that precisely controls chemokine levels and localization through high-affinity binding, operating independently of ligand-driven signaling. It acts as an interceptor, internalization receptor, and chemokine scavenger for CXCL11 and CXCL12/SDF1, inducing β-arrestin recruitment, ligand internalization, and MAPK pathway activation. ACKR3 Protein, Human (His-SUMO) is the recombinant human-derived ACKR3 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.

ACKR3 是一种非典型趋化因子受体，通过高亲和力趋化因子结合对趋化因子水平和定位进行精确控制，其运作独立于传统配体驱动的信号转导级联。作为拦截器、内化受体、趋化因子清除受体或趋化因子诱饵受体，ACKR3 与 CXCL11 和 CXCL12/SDF1 等趋化因子结合，而不激活 G 蛋白介导的信号转导。相反，趋化因子结合会诱导 β-抑制蛋白募集，导致配体内化和 MAPK 信号通路激活。在迁移的中间神经元中，ACKR3 对于调节 CXCR4 蛋白水平、调整其对趋化因子的反应至关重要。在神经胶质瘤细胞中，ACKR3 通过 MEK/ERK 途径转导信号，赋予细胞凋亡抵抗力并促进细胞生长和存活。虽然不影响正常造血祖细胞的迁移、粘附或增殖，但当恶性造血细胞中的 CXCL11 激活时，ACKR3 会通过 ERK1/2 磷酸化增强细胞粘附和迁移。此外，ACKR3 在 CXCR4 介导的细胞表面整合素激活中发挥调节作用，对心脏瓣膜发育至关重要。在动眼神经系统中，ACKR3 通过调节 CXCL12 水平来调节轴突引导。此外，作为 CXCR4 的共受体，ACKR3 在微生物感染期间与有限数量的 HIV 分离株合作。

Human

E. coli

N-SUMO;N-6*His

P25106 (M1-K40)

57007  [NCBI]

Extracellular Domain

MDLHLFDYSEPGNFSDISWPCNSSDCIVVDTVMCPNMPNK

Approximately 27 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH8.0 or PBS, pH 8.0, 6% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.