1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. Adenylate Kinase 1/AK1 蛋白, Rat (His)

Adenylate Kinase 1/AK1 蛋白, Rat (His)

目录号: HY-P74427
COA 产品使用指南 技术支持

腺苷酸激酶 1 (AK1) 蛋白通过催化 ATP 和 AMP 之间末端磷酸基团的可逆转移来维持腺苷酸核苷酸的平衡,从而在细胞能量稳态中发挥至关重要的作用。Adenylate Kinase 1/AK1 蛋白, Rat (His) 是重组的 腺苷酸激酶 AK1 蛋白,由 E. coli 表达,带有 N-His 标签。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥670 In-stock
10 μg ¥1080 In-stock
50 μg ¥2800 In-stock
100 μg ¥4500 In-stock
500 μg ¥11700 In-stock
1 mg ¥18700 询价
> 1 mg   询价  

* Please select Quantity before adding items.

Adenylate Kinase 1/AK1 Protein, Rat (His) 相关产品:

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The adenylate kinase 1 (AK1) protein plays a crucial role in cellular energy homeostasis by maintaining the balance of adenylate nucleotides by catalyzing the reversible transfer of terminal phosphate groups between ATP and AMP.Adenylate Kinase 1/AK1 Protein, Rat (His) is the recombinant rat-derived Adenylate Kinase 1/AK1 protein, expressed by E.coli , with N-His labeled tag.

研究背景

腺苷酸激酶 1 (AK1) 是一种多功能酶,可催化 ATP 和 AMP 之间末端磷酸基团的可逆转移,这是细胞能量稳态的关键步骤。此外,AK1 还具有核苷二磷酸激酶活性,可使用 ATP 或 GTP 作为磷酸盐供体,促进从相应的二磷酸底物生成各种核苷三磷酸(ATP、CTP、GTP、UTP、dATP、dCTP、dGTP 和 dTTP)。这种酶的灵活性凸显了其在核苷酸生物合成中的重要作用。此外,AK1 对从二磷酸硫胺素 (ThDP) 和 ADP 合成三磷酸硫胺素 (ThTP) 的催化速率较低,表明其参与硫胺素代谢。AK1 的多方面功能强调了其在维持细胞能量水平和参与多种核苷酸和辅因子生物合成途径中的重要性。参考资料:所提供的信息基于截至 2022 年 1 月的一般知识,未引用具体参考资料。

生物活性

Specific activity is 933.598 units/mg. One unit will convert ATP + AMP to 2.0 µmoles of ADP per minute at pH 7.5 at 37 °C.

检测步骤

Materials
Kinase-LumiTM Enhanced Chemiluminescent Kinase Assay Kit
Assay buffer: 40 mM Tris, 20 mM MgCl2, 0.1 mg/mL BSA, pH 7.4
Adenylate Kinase 1/AK1 Protein, Rat (His)(HY-P74427)
Substrate: AMP, ATP
Standard: ATP

Assay Procedure
Standard Curve Preparation
1. Dilute ATP (standard) in assay buffer to the following concentrations: 0, 0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40, 50 μM.
2. Add 50 μL of each standard concentration to a well and add 50 μL of Kinase-Lumi? Enhanced Chemiluminescent Kinase Assay Reagent.
3. Incubate at room temperature in the dark for 10 minutes.
4. Using a microplate reader, measure the RLU value under luminescence.
5. Plot the measured RLU on the x-axis and the standard concentration on the y-axis to generate a standard curve and obtain the standard curve equation.

Protein Activity Assay
1. Dilute AK1 to 10 μg/mL in assay buffer.
2. Dilute ATP to 40 μM (provided in the kit) and AMP to 40 μM in assay buffer to prepare the reaction mixture.
3. Add 25 μL of 10 μg/mL AK1 to a 96-well plate, then add 25 μL of the ATP-AMP mixture to start the reaction; for the blank, add 25 μL of assay buffer and 25 μL of the ATP-AK1 mixture.
4. Incubate at 37°C in the dark for 50 minutes.
5. After incubation, add 50 μL of Kinase-LumiTM Enhanced Chemiluminescent Kinase Assay Reagent to each well.
6. Incubate at room temperature for 10 minutes.
7. Using a microplate reader, measure the RLU value under luminescence.
8. Calculate the specific activity:

     Specific Activity (pmol/min/μg) =

Adjusted Vmax* (RFU/min) x Conversion Factor ** (pmol/RFU)
amount of enzyme (μg)

*Adjusted for Control
**Derived using calibration standard

Per Well:
Rat AK1: 0.25 μg
AMP: 20 μM
ATP: 20 μM

种属

Rat

表达系统

E. coli

标签

N-6*His

蛋白编号

P39069 (M1-K194)

基因 ID
蛋白结构
N-term
His
AK1 (M1-K194)
Accession # P39069
C-term
蛋白长度

Full Length

中文名
Adenylate 激酶 1 蛋白/AK1 蛋白
同用名
Adenylate kinase isoenzyme 1; ATP-AMP transphosphorylase 1; AK1
氨基酸序列

MEDKLKKAKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSSRGKMLSSIMEKGELVPLETVLDMLRDAMLAKVDSSNGFLIDGYPREVKQGEEFERKIAQPTLLLYVDAGPETMTQRLLKRGETSGRVDDNEETIKKRLETYYKATEPVISFYDKRGIVRKVNAEGSVDTVFSQVCTYLDSLK

分子量

Approximately 23 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

溶液.

组分

Supplied as a 0.22 μm filtered solution of PBS, 100 mM arginine, pH 7.1, 20% glycerol.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Adenylate Kinase 1/AK1 Protein, Rat (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Adenylate Kinase 1/AK1 Protein, Rat (His)
目录号:
HY-P74427
需求量: