beta-Catenin 蛋白, Mouse (sf9, His-GST)

β-Catenin is a key component of canonical Wnt signaling, which dynamically controls cellular processes. In the absence of Wnt, it forms a complex leading to phosphorylation and degradation. beta-Catenin Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived beta-Catenin protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of beta-Catenin Protein, Mouse (sf9, His-GST) is 781 a.a., with molecular weight of ~115 kDa.

β-连环蛋白是经典 Wnt 信号通路中的关键参与者，可响应 Wnt 配体动态调节细胞过程。在 Wnt 缺失的情况下，β-连环蛋白参与与 AXIN1、AXIN2、APC、CSNK1A1 和 GSK3B 的复合物，促进其磷酸化和泛素化，导致随后被蛋白酶体降解。Wnt 激活后，β-连环蛋白在细胞核中积聚，作为 TCF/LEF 家族转录因子的共激活剂，从而激活 Wnt 响应基因。这种多面蛋白还在细胞粘附中发挥作用，作为 E-钙粘蛋白：连环蛋白粘附复合物的组成部分。除了这些功能之外，β-连环蛋白还作为中心体凝聚力的负调节剂出现，并在胰岛素内化、抑制失巢凋亡和促进神经发生中发挥作用。它调节软骨细胞分化，在牙胚形成过程中积极调节成牙本质细胞分化，并与各种蛋白质相互作用，包括 Wnt 信号传导的调节子和效应子，突出其在多种细胞过程中的核心作用。β-连环蛋白复杂的相互作用网络强调了其在协调细胞对复杂信号线索的反应中的重要性，有助于调节关键的细胞功能。

Mouse

Sf9 insect cells

N-His;N-GST

Q02248 (M1-L781)

12387  [NCBI]

Approximately 115 kDa

Greater than 88% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.