Caspase-3/CASP3 蛋白, Human

Caspase-3/CASP3 protein is a thiol protease critical in apoptosis and is activated by initiating caspases (CASP8, CASP9 and/or CASP10). It catalyzes the cleavage of multiple proteins in sympathetic neurons, including PARP1, SREBP, caspase-6, -7 and -9, huntingtin, and RET. Caspase-3/CASP3 Protein, Human is the recombinant human-derived Caspase-3/CASP3 protein, expressed by E. coli , with tag free. The total length of Caspase-3/CASP3 Protein, Human is 248 a.a., .

Caspase-3/CASP3 蛋白在细胞凋亡执行阶段作为关键硫醇蛋白酶发挥作用，作为主要效应 caspase。在被起始半胱天冬酶（CASP8、CASP9 和/或 CASP10）裂解和激活后，它通过催化多种蛋白质的裂解来协调细胞凋亡。在细胞凋亡的早期阶段，它以蛋白水解方式裂解聚（ADP-核糖）聚合酶 PARP1，靶向“216-Asp-|-Gly-217”键。CASP3 还激活甾醇调节元件结合蛋白 (SREBP)，裂解并激活 caspase-6、-7 和 -9，参与亨廷顿蛋白的裂解，并通过 RET 裂解诱导交感神经元中的细胞粘附。此外，CASP3 会裂解并抑制丝氨酸/苏氨酸蛋白激酶 AKT1 以应对氧化应激。它通过裂解抗病毒蛋白 CGAS、IRF3 和 MAVS，在病毒诱导的细胞凋亡过程中充当 I 型干扰素产生的抑制剂，从而防止细胞因子过度产生。CASP3 还参与细胞焦亡，介导gasdermin-E (GSDME) 的裂解和激活。此外，它还能裂解 XRCC4 和磷脂扰乱酶蛋白 XKR4、XKR8 和 XKR9，促进凋亡细胞表面的磷脂酰丝氨酸暴露。

Measured by its ability to cleave the fluorogenic peptide substrate Ac-DEVD-AFC. The specific activity is ≥26570 pmol/min/µg, as measured under the described conditions.

Human

E. coli

Tag Free

P42574 (S29-D175&S176-H277)

836

Approximately 12.6 kDa&16.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.