PTPRC/CD45RABC 蛋白, Human (HEK293, His)

PTPRC/CD45RABC Protein, Human (HEK293, His) is a type 1 transmembrane protein tyrosine phosphatase (PTPase) expressed by all the haematopoietic stem cells (HSCs) except that of erythrocytes and platelets.

白细胞共同抗原，C型蛋白酪氨酸磷酸酶受体（PTPRC），也称为CD45，是一种跨膜糖蛋白，在除成熟红细胞外的几乎所有造血细胞上表达，是T和B细胞抗原受体介导的重要调节因子激活。CD45由两个胞质结构域、一个跨膜结构域和一个胞外结构域组成。蛋白酪氨酸激酶和磷酸酶活性（来自 CD45 等）之间的平衡破坏可能导致免疫缺陷、自身免疫或恶性肿瘤。CD45 通常存在于细胞表面，因此它在一个大型信号网络的上游发挥作用，而不同细胞类型的信号网络不同，因此 CD45 对这些细胞的影响也不同^[1]。

Measured by its ability to inhibit the proliferation of M-NFS-60 Mice myeloid leukemia lymphocytes. The ED₅₀ this effect is ≤2.406 μg/mL, corresponding to a specific activity is ≥415.62 units/mg.

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  Measured by its ability to inhibit the proliferation of M-NFS-60 Mice myeloid leukemia lymphocytes. The ED₅₀ for this effect is 1.932 μg/mL, corresponding to a specific activity is 517.5983 units/mg.

Human

HEK293

C-His

P08575-3 (Q26-K577)

5788  [NCBI]

Extracellular Domain

QSPTPSPTGLTTAKMPSVPLSSDPLPTHTTAFSPASTFERENDFSETTTSLSPDNTSTQVSPDSLDNASAFNTTGVSSVQTPHLPTHADSQTPSAGTDTQTFSGSAANAKLNPTPGSNAISDVPGERSTASTFPTDPVSPLTTTLSLAHHSSAALPARTSNTTITANTSDAYLNASETTTLSPSGSAVISTTTIATTPSKPTCDEKYANITVDYLYNKETKLFTAKLNVNENVECGNNTCTNNEVHNLTECKNASVSISHNSCTAPDKTLILDVPPGVEKFQLHDCTQVEKADTTICLKWKNIETFTCDTQNITYRFQCGNMIFDNKEIKLENLEPEHEYKCDSEILYNNHKFTNASKIIKTDFGSPGEPQIIFCRSEAAHQGVITWNPPQRSFHNFTLCYIKETEKDCLNLDKNLIKYDLQNLKPYTKYVLSLHAYIIAKVQRNGSAAMCHFTTKSAPPSQVWNMTVSMTSDNSMHVKCRPPRDRNGPHERYHLEVEAGNTLVRNESHKNCDFRVKDLQYSTDYTFKAYFHNGDYPGEPFILHHSTSYNSK

110-180 kDa

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  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4 or 50 mM Tris-HCl, 300 mM NaCl, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Maryam Ahmed Al Barashdi, et al. Protein tyrosine phosphatase receptor type C (PTPRC or CD45). J Clin Pathol. 2021 Sep;74(9):548-552.  [Content Brief]