CLEC1B/CLEC-2 蛋白, Mouse (HEK293, Fc)

The CLEC1B/CLEC-2 protein serves as a platelet receptor for PDPN. When activated, it signals through SRC and SYK kinases, activating PLCG2. CLEC1B forms homodimers and interacts with RACK1, promoting its ubiquitination and degradation. It also interacts with SYK and PDPN, independently of glycosylation, activating CLEC1B/CLEC-2. CLEC1B/CLEC-2 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CLEC1B/CLEC-2 protein, expressed by HEK293 , with N-hFc labeled tag. The total length of CLEC1B/CLEC-2 Protein, Mouse (HEK293, Fc) is 177 a.a., with molecular weight of 50-65 kDa.

CLEC1B/CLEC-2 蛋白是一种 C 型凝集素样受体，可作为 PDPN（淋巴内皮细胞标记物）的血小板受体。配体激活后，CLEC1B/CLEC-2 通过依次激活 SRC 和 SYK 酪氨酸激酶发出信号，最终导致 PLCG2 激活。它形成同二聚体并通过其胞质结构域与 RACK1 相互作用，促进 CLEC1B 泛素化并随后通过蛋白酶体途径降解。此外，CLEC1B/CLEC-2 在 SYK 的 SH2 结构域的促进下以二聚体形式与 SYK 相互作用。此外，它与 PDPN 相互作用，并且这种相互作用独立于 CLEC1B 糖基化，从而导致 CLEC1B/CLEC-2 的激活。

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Podoplanin is coated at 1.0 µg/mL (100 μL/well), Recombinant Mouse CLEC-2 binds with ED₅₀ of 0.2387 μg/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Mouse Podoplanin is coated at 1.0 µg/mL (100 μL/well), Recombinant Mouse CLEC‑2 binds with ED₅₀ of 0.2387 μg/mL.

Mouse

HEK293

N-hFc

Q9JL99-1 (M53-L229)

56760  [NCBI]

MSVTQQKYLLAEKENLSATLQQLAKKFCQELIRQSEIKTKSTFEHKCSPCATKWRYHGDSCYGFFRRNLTWEESKQYCTEQNATLVKTASQSTLDYIAERITSVRWIGLSRQNSKKDWMWEDSSVLRKNGINLSGNTEENMNCAYLHNGKIHPASCKERHYLICERNAGMTRVDQLL

Approximately 50-65 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.