1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. CSNK1G1 蛋白, Human (sf9, His-GST)

CSNK1G1 蛋白, Human (sf9, His-GST)

目录号: HY-P76850
产品使用指南

CSNK1G1 蛋白是酪蛋白激酶家族的成员,优先磷酸化酸性蛋白(包括酪蛋白),表现出广泛的底物特异性。在功能上,它积极参与 Wnt 信号通路并调节细胞反应。CSNK1G1 蛋白, Human (sf9, His-GST) 是重组的 CSNK1G1 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。CSNK1G1 蛋白, Human (sf9, His-GST) 全长 422 个氨基酸,分子量约为 ~66 kDa。

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描述

The CSNK1G1 protein is a member of the casein kinase family that preferentially phosphorylates acidic proteins, including casein, exhibiting broad substrate specificity. Functionally, it actively participates in the Wnt signaling pathway and regulates cellular responses. CSNK1G1 Protein, Human (sf9, His-GST) is the recombinant human-derived CSNK1G1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of CSNK1G1 Protein, Human (sf9, His-GST) is 422 a.a., with molecular weight of ~66 kDa.

研究背景

CSNK1G1 是一种属于酪蛋白激酶家族的丝氨酸/苏氨酸蛋白激酶,其操作定义涉及酸性蛋白(包括酪蛋白)的优先磷酸化。它具有广泛的底物特异性,能够磷酸化多种蛋白质。从功能上讲,CSNK1G1 积极参与复杂的 Wnt 信号通路,有助于调节细胞反应。此外,它在谷氨酸介导的突触信号的快速传递中发挥调节作用,证明它参与突触过程和神经递质信号传导,正如在酪蛋白激酶家族的其他成员中观察到的那样。值得注意的是,CSNK1G1 磷酸化 CLSPN,表明其在影响与 CLSPN 介导功能相关的细胞过程中的作用。

种属

Human

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

Q9HCP0-1 (M1-K422)

基因 ID
蛋白结构
N-term
His-GST
CSNK1G1 (M1-K422)
Accession # Q9HCP0
C-term
同用名
Casein kinase I isoform gamma-1; CKI-gamma 1
分子量

Approximately 66 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CSNK1G1 Protein, Human (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CSNK1G1 Protein, Human (sf9, His-GST)
目录号:
HY-P76850
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