EGFR 蛋白, Canine (sf9, His)

EGFR Protein is a receptor tyrosine kinase that binds to ligands of the EGF family. The high expression of EGFR protein can be used as a diagnostic marker for canine transitional cell carcinoma (TCC). EGFR Protein participate in the NF-kappa-B signaling pathway. The high expression of EGFR protein can be used as a diagnostic marker for canine transitional cell carcinoma (TCC). EGFR Protein, Canine (sf9, His) is the recombinant canine-derived EGFR protein, expressed by Sf9 insect cells , with C-His labeled tag.

EGFR 蛋白是一种受体酪氨酸激酶，与 EGF 家族的配体结合，包括 EGF、TGFA/ TGF-alpha、AREG、epigen/EPGN、BTC/betacellulin -纤维素蛋白、表调节蛋白/EREG 和 HBEGF/肝素结合 EGF。这种相互作用启动级联反应，将细胞外信号转化为细胞反应，包括受体同源和/或异源二聚化和关键细胞质残基的自磷酸化。磷酸化受体招募适配蛋白如 GRB2，激活下游信号级联，包括 RAS-RAF-MEK-ERK, PI3激酶-ATK, PLCgamma-PKC 和 STATs 模块。此外，EGFR 可能触发 NF-kappa-B 信号级联，并直接磷酸化 RGS16 等蛋白，激活其 GTPase 活性，可能将 EGF 受体信号传导与 G 蛋白偶联受体信号传导联系起来。此外，EGFR 磷酸化 MUC1，增强其与 SRC 和 CTNNB1/ β -catenin的相互作用。它通过与 CCDC88A/GIV 相互作用，正向调节细胞迁移，将 EGFR 保留在细胞膜配体刺激后，从而促进 EGFR 信号传导，触发细胞迁移。除了其典型功能外，EGFR 还有助于增强学习和记忆能力，并在哺乳动物疼痛信号传导中发挥作用，其异构体 2 可能作为 EGF 作用的拮抗剂。

Immobilized canine EGFR-His at 10 μg/mL (100 μL/well) can bind biotinylated canine His-EGF, The EC₅₀ of biotinylated canine His-EGF is 0.019-0.36 μg/mL.

Canine

Sf9 insect cells

C-His

XP_533073.3 (M1-S618)

404306  [NCBI]

Partial

MAVCQGTSNRLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYMQRNYDLSFLKTIQEVAGYVLIALNTVEKIPLENLQIIRGNVLYENTHALSVLSNYGSNKTGLQELPLRNLHEILQGAVRFSNNPVLCNVETIQWRDIVDNDFISNMSMDIQNQAGRCQKCDPSCPNGSCWGPGKENCQKLTKIICAQQCSGRCRGRSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACSSDSYEVEEDGVRKCKKCEGPCRKVCNGIGIGEFKDTLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTLPLDPKELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVGLNITSLGLRSLKEISDGDVIISGNRKLCYANTINWKKLFGTSSQKTKIINNKDEKACKAIGHVCHPLCSSEGCWGPGPRDCVSCRNVSRGKECVEKCNILEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDSCIKCAHYIDGPHCVKTCPAGIMGENNTLVWKFSDGSRMCHLCHPNCTYGCEGPGLEGCAKPGPKIPS

Approximately 87.2 kDa,based on SDS-PAGE under reducing conditions.

Greater than 90% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of 20 mM PB, 300 mM NaCl, 10% Glycerol, pH 7.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.