Eotaxin-3/CCL26 蛋白, Human (sf9)

Eotaxin-3/CCL26 Protein, Human (sf9) is a CC chemokine that binds to chemokine receptors CCR3 or CX3CR1 and is a chemotactic agent for eosinophils and basophils that mediates inflammatory responses. Eotaxin-3/CCL26 Protein, Human (sf9) is a recombinant human Eotaxin-3/CCL26 (M1-L94) protein expressed by Sf9 insect cells
^[1].

CCL26，也称为 Eotaxin-3、巨噬细胞炎症蛋白 4-α (MIP-4-α)，是 CC 趋化因子家族的一种小细胞因子，位于人类基因组中的第 7 号染色体上。它在多种组织中表达，包括心脏、肺和卵巢，以及细胞因子白细胞介素 4 刺激的内皮细胞。它对嗜酸性粒细胞和嗜碱性粒细胞具有趋化性，并通过与细胞表面趋化因子受体 CCR3 或 CX3CR1 结合而发挥作用。其中，CCR3是选择性表达于嗜酸性粒细胞、嗜碱性粒细胞和部分Th2细胞上的CCR。CCR3被认为与Th2介导的疾病相关，包括AD和哮喘，而CX3CR1与Th1介导的疾病相关，如类风湿性关节炎、糖尿病、扁平苔藓和牛皮癣。CCL26可能通过吸引CCR3表达细胞和CX3CR1表达细胞，在过敏和其他疾病（例如嗜酸性食管炎和Churg-Strauss综合征）的发病机制中发挥双重作用。此外，CCL26 还充当 CCR1、CCR2 和 CCR5^[1][2] 的天然拮抗剂。

CCL26 (1 nM, 18 h) induces eosinophil migration, where eosinophil migration is induced within 6 hours, then migration stabilizes until 12 hours and increases further to 18 hours with no increase after 18 hours. And CCR3 blockade completely inhibits eosinophil migration^[2].

CCL26 (human, 5 μg in 4 μL PBS, i.p.) induces calcium mobilization not only through CCR3 but also through CX3CR1 in a PTX-sensitive manner, and induces chemotactic. Besides, it can rapidly recruit mouse eosinophils and intravenously transferred human CD16+ NK cells into the peritoneal cavity in BALB/c mice^[3].

Human

Sf9 insect cells

Tag Free

Q9Y258 (M1-L94)

10344  [NCBI]

Approximately 8.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Marie-Chantal Larose, et al. Correlation between CCL26 production by human bronchial epithelial cells and airway eosinophils: Involvement in patients with severe eosinophilic asthma. J Allergy Clin Immunol. 2015 Oct;136(4):904-13.  [Content Brief]

  [2]. Véronique Provost, et al. CCL26/eotaxin-3 is more effective to induce the migration of eosinophils of asthmatics than CCL11/eotaxin-1 and CCL24/eotaxin-2. J Leukoc Biol. 2013 Aug;94(2):213-22.  [Content Brief]

  [3]. Takashi Nakayama, et al. Eotaxin-3/CC chemokine ligand 26 is a functional ligand for CX3CR1. J Immunol. 2010 Dec 1;185(11):6472-9.  [Content Brief]