1. 重组蛋白
  2. Cytokines and Growth Factors Receptor Proteins Enzymes & Regulators
  3. Ephrin/Eph Family Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. Eph Receptors
  5. EphB2
  6. EphB2 蛋白, Human (HEK293, Fc)

EphB2 蛋白, Human (HEK293, Fc)

目录号: HY-P70790
COA 产品使用指南

EphB2 蛋白是一种神经营养因子,是 eph 受体酪氨酸激酶的成员。EphB2 在癌症进展和免疫中发挥着重要作用。EphB2 可以直接与 NMDA 受体结合并诱导其酪氨酸磷酸化。EphB2 对于大脑中的神经元连接性和可塑性以及突触功能至关重要。此外,EphB2还可以与表达ephrinB配体的细胞相互作用,导致EphB2激活并磷酸化ephrinB细胞域中的酪氨酸。EphB2 还调节血栓形成和血块收缩[5]EphB2 蛋白, Human (HEK293, Fc) 是重组的 EphB2 蛋白,由 HEK293 表达,带有 C-hFc 标签。EphB2 蛋白, Human (HEK293, Fc) 全长 464 个氨基酸,分子量为 95-120 kDa。

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规格 价格 是否有货 数量
10 μg ¥1000 In-stock
50 μg ¥3000 In-stock
100 μg   询价  

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描述

EphB2 Protein is a neurotrophic factor, and a member of the eph receptor tyrosine kinase. EphB2 plays an important role in cancer progression and in immunity. EphB2 can bind to directly to the NMDA receptor and induces its tyrosine phosphorylation. EphB2 is essential for neuronal connectivity and plasticity in the brain, as well as synaptic function. Besides, EphB2 can also interact with cells expressing ephrinB ligands, and results in EphB2 activation and phosphorylation of tyrosines in the ephrinB cytodomain. EphB2 also regulates thrombus formation and clot retraction[1][2][3][4][5]. EphB2 Protein, Human (HEK293, Fc) is the recombinant human-derived EphB2 protein, expressed by HEK293, with C-hFc labeled tag. The total length of EphB2 Protein, Human (HEK293, Fc) is 464 a.a., with molecular weight of 95-120 kDa.

研究背景

EphB2 蛋白(Ephrin B 型受体 2)是一种神经营养因子,是 eph 受体酪氨酸激酶的成员。在大鼠肠道中,EphB2 优先由肠神经元表达[1]EphB2 在许多癌症中也异常表达,并在癌症进展中发挥重要作用。此外,EphB2在一些免疫细胞上表达,表明EphB2在免疫中的作用[2]
EphB2 可以直接与 NMDA 受体结合并诱导其酪氨酸磷酸化。据报道,EphB2 对于大脑中的神经元连接性和可塑性以及突触功能至关重要。EphB2 介导与学习新行为任务相关的神经元的结构可塑性[1][3]。此外,EphB2 还可与表达 ephrinB 配体的细胞相互作用,导致 EphB2 激活并磷酸化 ephrinB 细胞结构域中的酪氨酸[4]EphB2 在没有配体结合的情况下以接触无关的方式调节初始血小板活化,并且 EphB2 信号传导还调节血栓形成和凝块收缩[5]
EphB2 是一种大约 117 kDa 的跨膜蛋白。人类、小鼠和大鼠 EphB2 具有超过 98% 的氨基酸序列同一性。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

Q6NVW1 (V19-S482)

基因 ID
蛋白结构
N-term
EphB2 (V19-S482)
Accession # Q6NVW1
hFc
C-term
同用名
EPHB2 protein; EPHB2; Ephrin type-B receptor 2
氨基酸序列

VEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYEADFDSATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRSGFYLAFQDYGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKMKTQRS

分子量

95-120 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
EphB2 Protein, Human (HEK293, Fc)
目录号:
HY-P70790
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